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Performance and acceptability of the FluoLED Easy (TM) module for tuberculosis fluorescence microscopy

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dc.contributor.author Van Deun, A. en_US
dc.contributor.author Chonde, T. M. en_US
dc.contributor.author Gumusboga, M. en_US
dc.contributor.author Rienthong, S. en_US
dc.date.accessioned 2008-09-18T15:18:30Z
dc.date.available 2008-09-18T15:18:30Z
dc.date.issued 2008
dc.identifier.issn 1027-3719
dc.identifier.other ITG-M1A en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-MYCOB en_US
dc.identifier.other JIF en_US
dc.identifier.other UPD6 en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/2385
dc.description.abstract SETTING: Tuberculosis (TB) reference laboratory in Bangkok, Thailand, and two health centres in Dar es Salaam, Tanzania. OBJECTIVES: To assess the performance and user-friendliness of a light-emitting diode (LED) module (FluoLED Easy) for TB fluorescence microscopy (FM). DESIGN: Equivalence study vs. conventional FM in Bangkok using blinded re-reading; routine detection in the health centres in Dar es Salaam compared to Ziehl-Neelsen (ZN) over 2 years, with rechecking of FM smears. RESULTS: For 461 smears re-read, 99.1% concordance with conventional FM was obtained. FluoLED introduction caused a lasting increase in detection in the routine of each of the health centres by on average 20%. Blinded rechecking failed due to unreliable registration. Onsite rechecking of a convenience sample showed absence of false-positive results in one centre and confusion with artefacts that could have been avoided by more training in the other. LED FM was highly appreciated, with both laboratories refusing to revert to ZN as originally intended. CONCLUSIONS: A simple microscope with a FluoLED module can yield results equivalent to those of conventional FM. Low cost, technical appropriateness and excellent acceptance justify its use in low-income settings, contrary to classical systems. LED FM can lead to increased sensitivity, but for optimal yield good training and quality assurance remain essential requirements. en_US
dc.language English en_US
dc.publisher International Union Against Tuberculosis And Lung Disease
dc.subject Bacterial diseases en_US
dc.subject Tuberculosis en_US
dc.subject Bacterial diseases en_US
dc.subject Tuberculosis en_US
dc.subject Fluorescence microscopy en_US
dc.subject Ziehl-Neelsen smear en_US
dc.subject Auramine en_US
dc.subject LED module en_US
dc.subject Performance en_US
dc.subject Sensitivity en_US
dc.subject Quality assurance en_US
dc.subject User-friendliness en_US
dc.subject Acceptability en_US
dc.subject Cost en_US
dc.subject Developing countries en_US
dc.title Performance and acceptability of the FluoLED Easy (TM) module for tuberculosis fluorescence microscopy en_US
dc.type Article en_US
dc.citation.issue 9 en_US
dc.citation.jtitle International Journal of Tuberculosis and Lung Disease en_US
dc.citation.volume 12 en_US
dc.citation.pages 1009-1014 en_US
dc.publisher.place Paris
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/18713497
dc.citation.jabbreviation Int J Tuberc Lung Dis en_US


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