Institute of Tropical Medicine Antwerp
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Trypanosoma vivax: a simplified protocol for in vivo growth, isolation and cryopreservation

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dc.contributor.author Ndao, M. en_US
dc.contributor.author Magnus, E. en_US
dc.contributor.author Büscher, P. en_US
dc.contributor.author Geerts, S. en_US
dc.date.accessioned 2007-12-06T14:33:55Z
dc.date.available 2007-12-06T14:33:55Z
dc.date.issued 2004 en_US
dc.identifier.issn 1252-607X en_US
dc.identifier.other ITG-X1A en_US
dc.identifier.other ITG-P2B en_US
dc.identifier.other ITG-P3A en_US
dc.identifier.other ITG-ALA en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-SEROL en_US
dc.identifier.other ANIMAL en_US
dc.identifier.other U-ANIMAL en_US
dc.identifier.other JIF en_US
dc.identifier.other MULTI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/238
dc.description.abstract A rodent adapted clone of Trypanosoma vivax was used to infect cyclophosphamide treated mice and rats. Fresh blood containing trypanosomes, was centrifuged in a density gradient of three Percoll solutions, 1.07, 1.06, 1.05 g/ml, respectively, carefully layered on top of each other. The yields of this simple procedure for trypanosome purification were about six times higher than those obtained with the conventional anion-exchange columns. Cryopreservation of trypanosomes using glycerol yielded 90% viable parasites, whereas using dimethylsulfoxide, a more commonly used cryoprotectant, the viability was only 35%. en_US
dc.language English en_US
dc.subject Protozoal diseases en_US
dc.subject Trypanosoma vivax en_US
dc.subject Isolation and purification en_US
dc.subject Cyclophosphamide en_US
dc.subject Cryopreservation en_US
dc.subject Percoll en_US
dc.subject Laboratory techniques and procedures en_US
dc.subject Laboratory animals en_US
dc.subject Rodents en_US
dc.title Trypanosoma vivax: a simplified protocol for in vivo growth, isolation and cryopreservation en_US
dc.type Article en_US
dc.citation.jtitle Parasite en_US
dc.citation.volume 11 en_US
dc.citation.pages 103-106 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/15071835
dc.citation.jabbreviation Parasite en_US


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