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Molecular typing of the actin gene of Trichomonas vaginalis isolates by PCR-restriction fragment length polymorphism

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dc.contributor.author Crucitti, T.
dc.contributor.author Abdellati, S.
dc.contributor.author Van Dyck, E.
dc.contributor.author Buvé, A.
dc.date.accessioned 2008-10-28T16:02:36Z
dc.date.available 2008-10-28T16:02:36Z
dc.date.issued 2008
dc.identifier.issn 1198-743X
dc.identifier.doi http://dx.doi.org/10.1111/j.1469-0691.2008.02034.x
dc.identifier.other ITG-M1A
dc.identifier.other ITG-M2B
dc.identifier.other ITG-M3A
dc.identifier.other ITG-MLA
dc.identifier.other MICRO
dc.identifier.other U-HIVSTD
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other ABSTRACT
dc.identifier.other UPD7
dc.identifier.uri http://hdl.handle.net/10390/2414
dc.description The definitive version is available at www3.interscience.wiley.com en_US
dc.description.abstract Human trichomoniasis, caused by the protozoan Trichomonas vaginalis, is a highly prevalent sexually transmitted infection. However, little is known about the degree of strain variability of T. vaginalis. A reliable classification method for T. vaginalis strains would be a useful tool in the study of the epidemiology, pathogenesis and transmission of T. vaginalis. A PCR-restriction fragment length polymorphism typing method was designed and evaluated using T. vaginalis isolates obtained after culture of vaginal specimens collected in the Democratic Republic of Congo and in Zambia. The variation of the actin gene of T. vaginalis was determined for three ATCC reference strains and 151 T. vaginalis isolates. Eight different types were identified, on the basis of the digestion patterns of the amplified actin gene, with each of the restriction enzymes HindII, MseI and RsaI. It was determined that the ATCC reference strains 30001, 30240 and 50141 were of actin genotypes G, H and E, respectively. The actin genotype type E was more common in the Democratic Republic of Congo, whereas type G was the commonest type in Zambia. Translation of the nucleotide sequence showed up to three amino acid substitutions. We developed a reproducible, sensitive and specific typing method for T. vaginalis, and were able to distinguish at least eight T. vaginalis actin genotypes. Further studies are needed to evaluate the method using clinical specimens and to determine the utility of the typing method for the genotypic characterization of T. vaginalis. en_US
dc.language English en_US
dc.publisher Blackwell Publishing en_US
dc.subject Protozoology en_US
dc.subject Trichomonas vaginalis en_US
dc.subject Genotyping en_US
dc.subject PCR-RFLP en_US
dc.subject Actin gene en_US
dc.title Molecular typing of the actin gene of Trichomonas vaginalis isolates by PCR-restriction fragment length polymorphism en_US
dc.type Article en_US
dc.citation.issue 9 en_US
dc.citation.jtitle Clinical Microbiology and Infection en_US
dc.citation.volume 14 en_US
dc.citation.pages 844-852 en_US
dc.publisher.place Oxford en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/18844685
dc.citation.jabbreviation Clin Microbiol Infect en_US


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