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A Bayesian evaluation of three diagnostic assays for the detection of Giardia duodenalis in symptomatic and asymptomatic dogs

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dc.contributor.author Geurden, T.
dc.contributor.author Berkvens, D.
dc.contributor.author Casaert, S.
dc.contributor.author Vercruysse, J.
dc.contributor.author Claerebout, E.
dc.date.accessioned 2008-10-29T12:22:48Z
dc.date.available 2008-10-29T12:22:48Z
dc.date.issued 2008
dc.identifier.issn 0304-4017
dc.identifier.issn UPD7
dc.identifier.doi http://dx.doi.org/10.1016/j.vetpar.2008.07.002
dc.identifier.other ITG-A2A
dc.identifier.other ANIMAL
dc.identifier.other U-ANIMAL
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other ABSTRACT
dc.identifier.uri http://hdl.handle.net/10390/2421
dc.description.abstract A Bayesian approach was used to evaluate three commonly used diagnostic assays for the detection of Giardia duodenalis in dogs: microscopical examination (ME), a commercial immunofluorescence assay (IFA: MerifluorGiardia test) and a commercial immunochromatographic assay (SNAP: Idexx SNAPGiardia test). These assays were evaluated for use in two different settings: in a cross-sectional epidemiological survey in household dogs and in a clinical survey, both conducted in the northern part of Belgium. A total of 272 faecal samples from household dogs and 141 faecal samples from clinically affected dogs were examined using these three diagnostic assays. The Bayesian analysis indicated that all tests were highly specific (specificity above 90%), and that the IFA is more sensitive than SNAP and ME, both in an epidemiological and in a clinical setting. For all three tests, the estimated sensitivity values were higher in the clinical compared to the epidemiological survey, whereas the specificity values were comparable in both studies. The results of the present study indicate that IFA is a highly specific and sensitive technique for the detection of G. duodenalis cysts, both for use in an epidemiological or clinical survey. The SNAP is a specific and fairly sensitive technique for the diagnosis of Giardia in clinically affected dogs. Overall, the ME was found to be a specific diagnostic technique, although lacking sensitivity. en_US
dc.language English en_US
dc.publisher Elsevier
dc.subject Animal diseases en_US
dc.subject Dogs en_US
dc.subject Protozoal diseases en_US
dc.subject Giardia duodenalis en_US
dc.subject Laboratory diagnosis en_US
dc.subject Assays en_US
dc.subject Immunofluorescence en_US
dc.subject Immunochromatography en_US
dc.subject Microscopy en_US
dc.subject Bayes theorem en_US
dc.subject Evaluation studies en_US
dc.title A Bayesian evaluation of three diagnostic assays for the detection of Giardia duodenalis in symptomatic and asymptomatic dogs en_US
dc.type Article en_US
dc.citation.issue 1-2 en_US
dc.citation.jtitle Veterinary Parasitology en_US
dc.citation.volume 157 en_US
dc.citation.pages 14-20 en_US
dc.publisher.place Amsterdam
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/18723290
dc.identifier.url http://www.elsevier.com/locate/vetpar
dc.citation.jabbreviation Vet Parasitol en_US


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