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Rapid and inexpensive detection of multidrug-resistant Mycobacterium tuberculosis with the nitrate reductase assay using liquid medium and direct application to sputum samples

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dc.contributor.author Affolabi, D.
dc.contributor.author Odoun, M.
dc.contributor.author Sanoussi, N.
dc.contributor.author Martin, A.
dc.contributor.author Palomino, J. C.
dc.contributor.author Kestens, L.
dc.contributor.author Anagonou, S.
dc.contributor.author Portaels, F.
dc.date.accessioned 2008-10-29T13:30:36Z
dc.date.available 2008-10-29T13:30:36Z
dc.date.issued 2008
dc.identifier.issn 0095-1137
dc.identifier.doi http://dx.doi.org/10.1128/JCM.00083-08
dc.identifier.other ITG-M1B
dc.identifier.other ITG-M4A
dc.identifier.other ITG-M5A
dc.identifier.other ITG-M6A
dc.identifier.other ITG-MLA
dc.identifier.other MICRO
dc.identifier.other U-MYCOB
dc.identifier.other U-IMMUN
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other ABSTRACT
dc.identifier.other UPD7
dc.identifier.uri http://hdl.handle.net/10390/2426
dc.description.abstract For rapid and low-cost detection of multidrug-resistant (MDR) Mycobacterium tuberculosis, we applied the nitrate reductase assay (NRA) using a liquid medium directly to sputum samples. A total of 179 sputum samples were analyzed by the NRA, and results were compared to those obtained by the indirect proportion method (IPM) as a standard reference. Out of 144 specimens for which comparable results were available, only one discrepant result was obtained: MDR by NRA but susceptible by the IPM. In total 56% of the results were obtained in 10 days by the NRA. NRA performed in liquid medium is rapid and inexpensive and can be easily implemented in low-income countries. en_US
dc.language English en_US
dc.publisher American Society for Microbiology en_US
dc.subject Bacterial diseases en_US
dc.subject Tuberculosis en_US
dc.subject Mycobacterium tuberculosis en_US
dc.subject Multidrug resistance en_US
dc.subject Rapid diagnostic tests en_US
dc.subject Drug sensitivity testing en_US
dc.subject Nitrate reductase assay en_US
dc.subject Sputum en_US
dc.subject Affordability en_US
dc.subject Feasibility en_US
dc.subject Developing countries en_US
dc.title Rapid and inexpensive detection of multidrug-resistant Mycobacterium tuberculosis with the nitrate reductase assay using liquid medium and direct application to sputum samples en_US
dc.type Article en_US
dc.citation.issue 10 en_US
dc.citation.jtitle Journal of Clinical Microbiology en_US
dc.citation.volume 46 en_US
dc.citation.pages 3243-3245 en_US
dc.publisher.place Washington en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/18753346
dc.citation.jabbreviation J Clin Microbiol en_US


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