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Neutralization patterns and evolution of sequential HIV type 1 envelope sequences in HIV type 1 subtype B-infected drug-naive individuals

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dc.contributor.author Nyambi, P.
dc.contributor.author Burda, S.
dc.contributor.author Urbanski, M.
dc.contributor.author Heyndrickx, L.
dc.contributor.author Janssens, W.
dc.contributor.author Vanham, G.
dc.contributor.author Nadas, A.
dc.date.accessioned 2009-02-12T15:27:17Z
dc.date.available 2009-02-12T15:27:17Z
dc.date.issued 2008
dc.identifier.issn 0889-2229
dc.identifier.doi http://dx.doi.org/10.1089/aid.2008.0154
dc.identifier.other ITG-M4A
dc.identifier.other ITG-M5A
dc.identifier.other ITG-M6A
dc.identifier.other MICRO
dc.identifier.other U-VIROL
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other ABSTRACT
dc.identifier.other UPD9
dc.identifier.other FTA
dc.identifier.uri http://hdl.handle.net/10390/2498
dc.description.abstract To design a vaccine that will remain potent against HIV-1, the immunogenic regions in the viral envelope that tend to change as well as those that remain constant over time must be identified. To determine the neutralization profiles of sequential viruses over time and study whether neutralization patterns correlate with sequence evolution, 12 broadly neutralizing plasmas from HIV-1 subtype B-infected individuals were tested for their ability to neutralize sequential primary HIV-1 subtype B viruses from four individuals. Three patterns of neutralization were observed, including a loss of neutralization sensitivity by viruses over time, an increase in neutralization sensitivity by sequential viruses, or a similarity in the sensitivity of sequential viruses to neutralization. Seven to 11 gp160 clones from each sequential virus sample were sequenced and analyzed to identify mutational patterns. Analysis of the envelope sequences of the sequential viruses revealed changes characteristic of the neutralization patterns. Viruses that evolved to become resistant to neutralizing antibodies also evolved with diverse sequences, with most of the changes being due to nonsynonymous mutations occurring in the V1/V2, as well as in the constant regions (C2, C3, C4), the most changes occurring in the C3. Viruses from the patient that evolved to become more sensitive to neutralization exhibited less sequence diversity with fewer nonsynonymous changes that occurred mainly in the V1/V2 region. The V3 region remained constant over time for all the viruses tested. This study demonstrates that as viruses evolve in their host, they either become sensitive or resistant to neutralization by antibodies in heterologous plasma and mutations in different envelope regions account for these changes in their neutralization profiles. en
dc.language English en
dc.subject Virology en
dc.subject HIV-1 en
dc.subject Subtype B en
dc.subject Envelope en
dc.subject Sequence evolution en
dc.subject Mutations en
dc.subject Neutralization en
dc.subject Sensitivity en
dc.subject Resistance en
dc.title Neutralization patterns and evolution of sequential HIV type 1 envelope sequences in HIV type 1 subtype B-infected drug-naive individuals en
dc.type Article en
dc.citation.issue 12 en
dc.citation.jtitle AIDS Research and Human Retroviruses en
dc.citation.volume 24 en
dc.citation.pages 1507-1519 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/19018670
dc.citation.jabbreviation AIDS Res Hum Retroviruses en


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