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Fluorogenic assay for molecular typing of the Leishmania donovani complex: taxonomic and clinical applications

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Show simple item record Quispe Tintaya, K. W. en_US Laurent, T. en_US Decuypere, S. en_US Hide, M. en_US Bañuls, A. L. en_US De Doncker, S. en_US Rijal, S. en_US Cañavate, C. en_US Campino, L. en_US Dujardin, J. C. en_US 2007-12-06T14:34:01Z 2007-12-06T14:34:01Z 2005 en_US
dc.identifier.issn 0022-1899 en_US
dc.identifier.other ITG-P1B en_US
dc.identifier.other ITG-P2B en_US
dc.identifier.other ITG-P3B en_US
dc.identifier.other ITG-P6B en_US
dc.identifier.other ITG-PLA en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-PROTO en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other FTA en_US
dc.identifier.other ABSTRACT en_US
dc.description.abstract We describe a new fluorogenic assay for the identification of species and intraspecies groups within the Leishmania donovani complex. The assay combined (1) 2 polymerase chain reactions targeting the 2 cysteine proteinase b isogenes and (2) a fluorescence-resonance energy transfer/melting curve analysis of the polymorphisms within a 31-nt region. All strains within the L. donovani complex were distinguished from L. tropica, L. major, and L. aethiopica, and 5 distinct groups were identified within the L. donovani complex. Discrepancies were observed with the present taxonomy on the basis of isoenzyme analysis and concerned East African strains, which suggests the need for a systematic reevaluation of the taxonomy. The capacity to type parasites directly from clinical samples was demonstrated with blood and bone marrow samples. This rapid and high-throughput alternative for molecular diagnosis and epidemiological studies of visceral leishmaniasis could be adapted for use with other Leishmania species. en_US
dc.language English en_US
dc.publisher University of Chicago Press en_US
dc.subject Protozoology en_US
dc.subject Leishmania donovani en_US
dc.subject Strains en_US
dc.subject Species en_US
dc.subject Identification en_US
dc.subject Taxonomy en_US
dc.subject Molecular diagnostic techniques en_US
dc.subject Rapid tests en_US
dc.title Fluorogenic assay for molecular typing of the Leishmania donovani complex: taxonomic and clinical applications en_US
dc.type Article en_US
dc.citation.issue 4 en_US
dc.citation.jtitle Journal of Infectious Diseases en_US
dc.citation.volume 192 en_US
dc.citation.pages 685-692 en_US Chicago en_US
dc.citation.jabbreviation J Infect Dis en_US

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