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Antigen genes for molecular epidemiology of leishmaniasis: polymorphism of cysteine proteinase B and surface metalloprotease glycoprotein 63 in the Leishmania donovani complex

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dc.contributor.author Quispe Tintaya, K. W. en_US
dc.contributor.author Ying, X. en_US
dc.contributor.author Dedet, J. P. en_US
dc.contributor.author Rijal, S. en_US
dc.contributor.author De Bolle, X. en_US
dc.contributor.author Dujardin, J. C. en_US
dc.date.accessioned 2007-12-06T14:34:01Z
dc.date.available 2007-12-06T14:34:01Z
dc.date.issued 2004 en_US
dc.identifier.issn 0022-1899 en_US
dc.identifier.doi http://dx.doi.org/10.1086/382049
dc.identifier.other ITG-P1B en_US
dc.identifier.other ITG-P2B en_US
dc.identifier.other ITG-PLA en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-PROTO en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other FTA en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/255
dc.description.abstract BACKGROUND: Efficient monitoring of endemic and resurgent visceral leishmaniasis (VL) requires discriminatory molecular tools that allow direct characterization of etiological agents (i.e., the Leishmania donovani complex) in host tissues. This characterization is possible through restriction fragment-length polymorphism (RFLP) analysis of polymerase chain reaction (PCR)-amplified sequences (PCR-RFLP). METHODS: We present 2 new PCR-RFLP assays that target the gene locus of cysteine proteinase B (cpb), an important Leishmania antigen. The assays were applied to the characterization of 15 reference strains of the L. donovani complex, and their discriminatory power was compared with that of PCR-RFLP analysis of the gp63 gene, another Leishmania antigen, and with that of multilocus enzyme electrophoresis (MLEE), which is the reference standard for parasite typing. RESULTS: Restriction patterns of the cpb locus were polymorphic, but less so than gp63 patterns. When data for both loci were combined, differences between PCR-RFLP and MLEE results were encountered. Antigen gene analysis was more discriminatory and supported a different classification of parasites, one that fitted with their geographic origin. PCR-RFLP analysis of cpb also allowed direct genotyping of parasites in bone marrow aspirate and venous blood samples obtained from patients with VL. CONCLUSION: Antigen genes constitute valid targets for PCR-based Leishmania typing without the need for isolation of parasites. en_US
dc.language English en_US
dc.publisher University of Chicago Press en_US
dc.subject Protozoal diseases en_US
dc.subject Leishmaniasis en_US
dc.subject Visceral en_US
dc.subject Leishmania donovani en_US
dc.subject Molecular epidemiology en_US
dc.subject Polymorphism en_US
dc.title Antigen genes for molecular epidemiology of leishmaniasis: polymorphism of cysteine proteinase B and surface metalloprotease glycoprotein 63 in the Leishmania donovani complex en_US
dc.type Article en_US
dc.citation.issue 6 en_US
dc.citation.jtitle Journal of Infectious Diseases en_US
dc.citation.volume 189 en_US
dc.citation.pages 1035-1043 en_US
dc.publisher.place Chicago en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/14999607
dc.citation.jabbreviation J Infect Dis en_US


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