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Detection of Taenia solium antigens and anti-T. solium antibodies in paired serum and cerebrospinal fluid samples from patients with intraparenchymal or extraparenchymal neurocysticercosis

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dc.contributor.author Rodriguez, S.
dc.contributor.author Dorny, P.
dc.contributor.author Tsang, V. C. W.
dc.contributor.author Pretell, E. J.
dc.contributor.author Brandt, J.
dc.contributor.author Lescano, A. G.
dc.contributor.author Gonzalez, A. E.
dc.contributor.author Gilman, R. H.
dc.contributor.author Garcia, H. H.
dc.date.accessioned 2009-05-16T07:39:56Z
dc.date.available 2009-05-16T07:39:56Z
dc.date.issued 2009
dc.identifier.issn 0022-1899
dc.identifier.doi http://dx.doi.org/10.1086/597757
dc.identifier.other ITG-A2A
dc.identifier.other ITG-X5A
dc.identifier.other ANIMAL
dc.identifier.other U-ANIMAL
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other UPD13
dc.identifier.other FTA
dc.identifier.other ABSTRACT
dc.identifier.uri http://hdl.handle.net/10390/2633
dc.description.abstract Background. Neurocysticercosis (NCC) is a frequent cause of epilepsy worldwide. Compared with the more common parenchymal brain cysts, extraparenchymal infections are difficult to manage and have a poor prognosis. Serological assays are used to detect circulating Taenia solium antigens or anti-T. solium antibodies in serum or cerebrospinal fluid (CSF) samples. There are no guidelines on whether to use serum or CSF specimens for a particular assay. Methods. We obtained paired serum and CSF samples from 91 patients with NCC (48 had intraparenchymal NCC, and 43 had extraparenchymal NCC) for detection of antibodies, using an enzyme-linked immunotransfer blot (EITB) assay, and antigens, using a monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA). Results. For the intraparenchymal NCC group, the EITB assay yielded more true-positive results for serum samples, and the ELISA yielded slightly more true-positive results for CSF samples than for serum samples, but none of these differences were statistically significant. Most patients with calcified NCC were antibody positive but antigen negative. For extraparenchymal disease, all samples were antibody positive, and all but 2 were antigen positive, with most samples containing high antigen levels. Conclusions. The sensitivity of antibody-detecting EITB assays is not increased through the use of CSF samples rather than serum samples. The antigen-detecting ELISA performed better for CSF samples than for serum samples, but for both specimen types it was less sensitive than the EITB assay. Active and inactive NCC are better differentiated from each other by the antigen-detecting ELISA, for both serum and CSF samples. High antigen levels suggest the presence of subarachnoid NCC. en
dc.language English en
dc.publisher University of Chicago Press en
dc.subject Helminthic diseases en
dc.subject Neurocysticercosis en
dc.subject Laboratory diagnosis en
dc.subject Antibody detection en
dc.subject Antigen detection en
dc.subject Serum en
dc.subject Cerebrospinal fluid en
dc.subject Performance en
dc.subject Comparative study en
dc.subject Peru en
dc.subject America, Latin en
dc.title Detection of Taenia solium antigens and anti-T. solium antibodies in paired serum and cerebrospinal fluid samples from patients with intraparenchymal or extraparenchymal neurocysticercosis en
dc.type Article en
dc.citation.issue 9 en
dc.citation.jtitle Journal of Infectious Diseases en
dc.citation.volume 199 en
dc.citation.pages 1345-1352 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/19358669
dc.citation.jabbreviation J Infect Dis en


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