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A combined CXCL10, CXCL8 and H-FABP panel for the staging of human African trypanosomiasis patients

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dc.contributor.author Hainard, A.
dc.contributor.author Tiberti, N.
dc.contributor.author Robin, X.
dc.contributor.author Lejon, V.
dc.contributor.author Ngoyi, D. M.
dc.contributor.author Matovu, E.
dc.contributor.author Enyaru, J. C.
dc.contributor.author Fouda, C.
dc.contributor.author Ndung'u, J. M.
dc.contributor.author Lisacek, F.
dc.contributor.author Müller, M.
dc.contributor.author Turck, N.
dc.contributor.author Sanchez, J. C.
dc.date.accessioned 2009-08-14T14:45:13Z
dc.date.available 2009-08-14T14:45:13Z
dc.date.issued 2009
dc.identifier.issn 1935-2735
dc.identifier.doi http://dx.doi.org/10.1371/journal.pntd.0000459
dc.identifier.other ITG-P4A
dc.identifier.other PARAS
dc.identifier.other U-SEROL
dc.identifier.other JIF
dc.identifier.other ELECTRONIC
dc.identifier.other DOI
dc.identifier.other UPD16
dc.identifier.other ABSTRACT
dc.identifier.other FTA
dc.identifier.uri http://hdl.handle.net/10390/2737
dc.description.abstract BACKGROUND: Human African trypanosomiasis (HAT), also known as sleeping sickness, is a parasitic tropical disease. It progresses from the first, haemolymphatic stage to a neurological second stage due to invasion of parasites into the central nervous system (CNS). As treatment depends on the stage of disease, there is a critical need for tools that efficiently discriminate the two stages of HAT. We hypothesized that markers of brain damage discovered by proteomic strategies and inflammation-related proteins could individually or in combination indicate the CNS invasion by the parasite. METHODS: Cerebrospinal fluid (CSF) originated from parasitologically confirmed Trypanosoma brucei gambiense patients. Patients were staged on the basis of CSF white blood cell (WBC) count and presence of parasites in CSF. One hundred samples were analysed: 21 from stage 1 (no trypanosomes in CSF and </=5 WBC/microL) and 79 from stage 2 (trypanosomes in CSF and/or >5 WBC/microL) patients. The concentration of H-FABP, GSTP-1 and S100beta in CSF was measured by ELISA. The levels of thirteen inflammation-related proteins (IL-1ra, IL-1beta, IL-6, IL-9, IL-10, G-CSF, VEGF, IFN-gamma, TNF-alpha, CCL2, CCL4, CXCL8 and CXCL10) were determined by bead suspension arrays. RESULTS: CXCL10 most accurately distinguished stage 1 and stage 2 patients, with a sensitivity of 84% and specificity of 100%. Rule Induction Like (RIL) analysis defined a panel characterized by CXCL10, CXCL8 and H-FABP that improved the detection of stage 2 patients to 97% sensitivity and 100% specificity. CONCLUSION: This study highlights the value of CXCL10 as a single biomarker for staging T. b. gambiense-infected HAT patients. Further combination of CXCL10 with H-FABP and CXCL8 results in a panel that efficiently rules in stage 2 HAT patients. As these molecules could potentially be markers of other CNS infections and disorders, these results should be validated in a larger multi-centric cohort including other inflammatory diseases such as cerebral malaria and active tuberculosis. en
dc.language English en
dc.subject Protozoal diseases en
dc.subject Trypanosomiasis, African en
dc.subject Stage determination en
dc.subject Markers en
dc.subject Sensitivity en
dc.subject Specificity en
dc.subject Evaluation en
dc.title A combined CXCL10, CXCL8 and H-FABP panel for the staging of human African trypanosomiasis patients en
dc.type Article-E en
dc.citation.issue 6 en
dc.citation.jtitle PLoS Neglected Tropical Diseases en
dc.citation.volume 3 en
dc.citation.pages e459 en
dc.citation.jabbreviation PLoS Negl Trop Dis en


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