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Bioluminescent imaging of Trypanosoma brucei shows preferential testis dissemination which may hamper drug efficacy in sleeping sickness

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dc.contributor.author Claes, F.
dc.contributor.author Vodnala, S. K.
dc.contributor.author van Reet, N.
dc.contributor.author Boucher, N.
dc.contributor.author Lunden-Miguel, H.
dc.contributor.author Baltz, T.
dc.contributor.author Goddeeris, B. M.
dc.contributor.author Büscher, P.
dc.contributor.author Rottenberg, M. E.
dc.date.accessioned 2009-08-24T07:36:55Z
dc.date.available 2009-08-24T07:36:55Z
dc.date.issued 2009
dc.identifier.issn 1935-2735
dc.identifier.doi http://dx.doi.org/10.1371/journal.pntd.0000486
dc.identifier.other ITG-P1A
dc.identifier.other ITG-P3B
dc.identifier.other ITG-P8A
dc.identifier.other PARAS
dc.identifier.other U-SEROL
dc.identifier.other JIF
dc.identifier.other ELECTRONIC
dc.identifier.other DOI
dc.identifier.other UPD16
dc.identifier.other ABSTRACT
dc.identifier.other FTA
dc.identifier.uri http://hdl.handle.net/10390/2741
dc.description.abstract Monitoring Trypanosoma spread using real-time imaging in vivo provides a fast method to evaluate parasite distribution especially in immunoprivileged locations. Here, we generated monomorphic and pleomorphic recombinant Trypanosoma brucei expressing the Renilla luciferase. In vitro luciferase activity measurements confirmed the uptake of the coelenterazine substrate by live parasites and light emission. We further validated the use of Renilla luciferase-tagged trypanosomes for real-time bioluminescent in vivo analysis. Interestingly, a preferential testis tropism was observed with both the monomorphic and pleomorphic recombinants. This is of importance when considering trypanocidal drug development, since parasites might be protected from many drugs by the blood-testis barrier. This hypothesis was supported by our final study of the efficacy of treatment with trypanocidal drugs in T. brucei-infected mice. We showed that parasites located in the testis, as compared to those located in the abdominal cavity, were not readily cleared by the drugs. en
dc.language English en
dc.subject Protozoal diseases en
dc.subject Trypanosomiasis, African en
dc.subject Trypanosoma brucei en
dc.subject Parasite detection en
dc.subject Imaging en
dc.subject Bioluminescence en
dc.subject Dissemination en
dc.subject Testicular en
dc.subject Drug therapy en
dc.subject Trypanocidal agents en
dc.subject Efficacy en
dc.subject Mice en
dc.title Bioluminescent imaging of Trypanosoma brucei shows preferential testis dissemination which may hamper drug efficacy in sleeping sickness en
dc.type Article-E en
dc.citation.issue 7 en
dc.citation.jtitle PLoS Neglected Tropical Diseases en
dc.citation.volume 3 en
dc.citation.pages e486 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/19621071
dc.citation.jabbreviation PLoS Negl Trop Dis en


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