Institute of Tropical Medicine Antwerp
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Towards a new reference test for surra in camels

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Show simple item record Tran, T. Claes, F. Verloo, D. De Greve, H. Büscher, P. 2009-08-31T07:42:05Z 2009-08-31T07:42:05Z 2009
dc.identifier.issn 1556-6811
dc.identifier.issn ABSTRACT
dc.identifier.other ITG-P1B
dc.identifier.other ITG-P2A
dc.identifier.other ITG-PLA
dc.identifier.other PARAS
dc.identifier.other U-SEROL
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other UPD16
dc.description.abstract Current serological diagnosis of Trypanosoma evansi infection in camels is based on the native variable antigen type RoTat 1.2. The goal of this study was to develop a novel serological diagnostic test based on a nonvariable protein and freed from the use of rats or mice for its production. An enzyme-linked immunosorbent assay using a recombinant extracellular domain of invariant surface glycoprotein 75 (ELISA/rISG75) was developed and tested on a collection of 184 camel sera. The results were compared to those obtained from three established antibody detection tests based on variable surface glycoprotein RoTat 1.2: an ELISA for T. evansi (ELISA/T. evansi), a card agglutination test for trypanosomiasis (CATT/T. evansi), and an immune trypanolysis (TL) assay. The ELISA/rISG75 and the ELISA/T. evansi showed a sensitivity of 94.6% (95% confidence interval [CI], 87.8 to 98.2%, at 19% positivity cutoff value) and 98.9% (95% CI, 94.1 to 99.8, at 12% positivity cutoff value), respectively. The ELISA/rISG75 had 100% specificity (CI, 95.9 to 100%), while the ELISA/T. evansi showed 98.9% specificity (CI, 95.9 to 100%). The ELISA/rISG75 demonstrated an almost perfect agreement with the TL assay, the CATT/T. evansi, and the ELISA/T. evansi, with kappa scores of at least 0.94. The ELISA/rISG75, having a performance comparable to that of the gold standard (the TL assay) and being independent of antigenic variation, may become a new reference test for surra in camels. It opens avenues for the diagnosis of T. evansi infections in other hosts as well as for the development of a pan-Trypanozoon test for detection of Trypanosoma brucei brucei, T. b. gambiense, T. b. rhodesiense, T. evansi, and T. equiperdum. en
dc.language English en
dc.subject Animal diseases en
dc.subject Camels en
dc.subject Protozoal diseases en
dc.subject Trypanosomiasis, African en
dc.subject Surra en
dc.subject Trypanosoma evansi en
dc.subject Laboratory diagnosis en
dc.subject Serodiagnosis en
dc.subject ELISA en
dc.subject Surface glycoproteins en
dc.subject Invariant en
dc.title Towards a new reference test for surra in camels en
dc.type Article en
dc.citation.issue 7 en
dc.citation.jtitle Clinical and Vaccine Immunology en
dc.citation.volume 16 en
dc.citation.pages 999-1002 en
dc.citation.jabbreviation Clin Vaccine Immunol en

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