Institute of Tropical Medicine Antwerp
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Standardization of the egg hatch test for the detection of benzimidazole resistance in parasitic nematodes

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dc.contributor.author von Samson-Himmelstjerna, G.
dc.contributor.author Coles, G. C.
dc.contributor.author Jackson, F.
dc.contributor.author Bauer, C.
dc.contributor.author Borgsteede, F.
dc.contributor.author Cirak, V. Y.
dc.contributor.author Demeler, J.
dc.contributor.author Donnan, A.
dc.contributor.author Dorny, P.
dc.contributor.author Epe, C.
dc.contributor.author Harder, A.
dc.contributor.author Höglund, J.
dc.contributor.author Kaminsky, R.
dc.contributor.author Kerboeuf, D.
dc.contributor.author Küttler, U.
dc.contributor.author Papadopoulos, E.
dc.contributor.author Posedi, J.
dc.contributor.author Small, J.
dc.contributor.author Várady, M.
dc.contributor.author Vercruysse, J.
dc.contributor.author Wirtherle, N.
dc.date.accessioned 2009-09-07T11:48:01Z
dc.date.available 2009-09-07T11:48:01Z
dc.date.issued 2009
dc.identifier.issn 0932-0113
dc.identifier.doi http://dx.doi.org/10.1007/s00436-009-1466-1
dc.identifier.other ITG-A9A
dc.identifier.other ANIMAL
dc.identifier.other U-ANIMAL
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other NOT AVAILABLE
dc.identifier.other UPD16
dc.identifier.other ABSTRACT
dc.identifier.uri http://hdl.handle.net/10390/2785
dc.description.abstract The ability to reliably detect anthelmintic resistance is a crucial part of resistance management. If data between countries are to be compared, the same test should give the same results in each laboratory. As the egg hatch test for benzimidazole resistance is used for both research and surveys, the ability of different laboratories to obtain similar results was studied through testing of known isolates of cyathostomins, Haemonchus contortus, Ostertagia ostertagi, and Cooperia oncophora in programs supported by the EU (Cost B16 and FP6-PARASOL). Initial results showed difficulties in obtaining reproducible and similar data within and between laboratories. A series of ring tests, i.e., simultaneous and coordinated rounds of testing of nematode isolates in different laboratories was subsequently performed. By adopting identical protocols, especially the use of deionized water and making dilutions of thiabendazole in dimethyl sulfoxide in the final ring test, laboratories correctly identified both susceptible and resistant isolates. The protocols for the test and preparation of solutions of thiabendazole are described. en
dc.language English en
dc.subject Helminthic diseases en
dc.subject Nematode infections en
dc.subject Anthelmintics en
dc.subject Benzimidazoles en
dc.subject Drug sensitivity testing en
dc.subject Standardization en
dc.subject Egg hatch test en
dc.title Standardization of the egg hatch test for the detection of benzimidazole resistance in parasitic nematodes en
dc.type Article en
dc.citation.issue 3 en
dc.citation.jtitle Parasitology Research en
dc.citation.volume 105 en
dc.citation.pages 825-834 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/19452165
dc.citation.jabbreviation Parasitol Res en


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