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Leishmania OligoC-TesT as a simple, rapid, and standardized tool for molecular diagnosis of cutaneous leishmaniasis in Peru

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dc.contributor.author Espinosa, D.
dc.contributor.author Boggild, A. K.
dc.contributor.author Deborggraeve, S.
dc.contributor.author Laurent, T.
dc.contributor.author Valencia, C.
dc.contributor.author Pacheco, R.
dc.contributor.author Miranda-Verástegui, C.
dc.contributor.author Llanos-Cuentas, A.
dc.contributor.author Leclipteux, T.
dc.contributor.author Dujardin, J. C.
dc.contributor.author Büscher, P.
dc.contributor.author Arévalo, J.
dc.date.accessioned 2009-09-07T12:46:58Z
dc.date.available 2009-09-07T12:46:58Z
dc.date.issued 2009
dc.identifier.issn 0095-1137
dc.identifier.doi http://dx.doi.org/10.1128/JCM.00259-09
dc.identifier.other ITG-P3B
dc.identifier.other ITG-P10A
dc.identifier.other ITG-P11A
dc.identifier.other PARAS
dc.identifier.other U-SEROL
dc.identifier.other U-PROTO
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other UPD16
dc.identifier.other ABSTRACT
dc.identifier.uri http://hdl.handle.net/10390/2791
dc.description.abstract Molecular methods such as PCR have become attractive tools for diagnosis of cutaneous leishmaniasis (CL), both for their high sensitivity and for their specificity. However, their practical use in routine diagnosis is limited due to the infrastructural requirements and the lack of any standardization. Recently, a simplified and standardized PCR format for molecular detection of Leishmania was developed. The Leishmania OligoC-TesT is based on simple and rapid detection using a dipstick with PCR-amplified Leishmania DNA. In this study, we estimated the diagnostic accuracy of the Leishmania OligoC-TesT for 61 specimens from 44 CL-suspected patients presenting at the leishmaniasis clinic of the Instituto de Medicina Tropical Alexander von Humboldt, Peru. On the basis of parasitological detection and the leishmanin skin test (LST), patients were classified as (i) confirmed CL cases, (ii) LST-positive cases, and (iii) LST-negative cases. The sensitivities of the Leishmania OligoC-TesT was 74% (95% confidence interval (CI), 60.5% to 84.1%) for lesion aspirates and 92% (95% CI, 81.2% to 96.9%) for scrapings. A significantly higher sensitivity was observed with a conventional PCR targeting the kinetoplast DNA on the aspirates (94%) (P = 0.001), while there was no significant difference in sensitivity for the lesion scrapings (88%) (P = 0.317). In addition, the Leishmania OligoC-TesT was evaluated for 13 CL-suspected patients in two different peripheral health centers in the central jungle of Peru. Our findings clearly indicate the high accuracy of the Leishmania OligoC-TesT for lesion scrapings for simple and rapid molecular diagnosis of CL in Peru. en
dc.language English en
dc.subject Protozoal diseases en
dc.subject Leishmaniasis en
dc.subject Cutaneous en
dc.subject Molecular diagnostic techniques en
dc.subject PCR en
dc.subject Rapid diagnostic tests en
dc.subject Dipstick en
dc.subject Standardization en
dc.subject Sensitivity en
dc.subject Accuracy en
dc.subject Evaluation studies en
dc.subject Peru en
dc.subject America, Latin en
dc.title Leishmania OligoC-TesT as a simple, rapid, and standardized tool for molecular diagnosis of cutaneous leishmaniasis in Peru en
dc.type Article en
dc.citation.issue 8 en
dc.citation.jtitle Journal of Clinical Microbiology en
dc.citation.volume 47 en
dc.citation.pages 2560-2563 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/19553579
dc.citation.jabbreviation J Clin Microbiol en


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