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Expression and role of CXCL10 during the encephalitic stage of experimental and clinical African trypanosomiasis

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dc.contributor.author Amin, D. N.
dc.contributor.author Rottenberg, M. E.
dc.contributor.author Thomsen, A. R.
dc.contributor.author Mumba, D.
dc.contributor.author Fenger, C.
dc.contributor.author Kristensson, K.
dc.contributor.author Büscher, P.
dc.contributor.author Finsen, B.
dc.contributor.author Masocha, W.
dc.date.accessioned 2009-12-18T12:16:40Z
dc.date.available 2009-12-18T12:16:40Z
dc.date.issued 2009
dc.identifier.issn 0022-1899
dc.identifier.doi http://dx.doi.org/10.1086/644597
dc.identifier.other ITG-P7A
dc.identifier.other PARAS
dc.identifier.other U-SEROL
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other UPD17
dc.identifier.other ABSTRACT
dc.identifier.other FTA
dc.identifier.uri http://hdl.handle.net/10390/2844
dc.description.abstract BACKGROUND: Human African trypanosomiasis, caused by Trypanosoma brucei, involves an early hemolymphatic stage followed by a late encephalitic stage. METHODS: We studied the expression of chemokines with use of microarray and enzyme-linked immunosorbent assay in T. brucei brucei-infected mice and in patients with human African trypanosomiasis and examined their role in controlling brain accumulation of T cells and parasites. RESULTS: The messenger RNAs (mRNAs) encoding CXCR3 ligands CXCL9 and CXCL10 demonstrated the greatest increases among chemokines in brain specimens of infected mice, as determined by microarray. CXCL9 and CXCL10 mRNA accumulation was interferon (IFN)-gamma-dependent. Expression of CXCL10 was predominantly observed in astrocytes. Weight loss was registered in wild-type but not in CXCL10(-/-) and CXCR3(-/-) infected mice. Infected CXCL10(-/-) or CXCR3(-/-) mice demonstrated reduced accumulation of trypanosomes and T cells in the brain parenchyma but similar parasitemia levels, compared with wild-type mice. CXCL10 and IFN-gamma levels were increased in the cerebrospinal fluid of patients with late stage but not early stage human African trypanosomiasis. Levels of CXCL10 in patients with late stage human African trypanosomiasis were associated with somnolence, low body weight, and trypanosomes in the cerebrospinal fluid. CONCLUSION: IFN-gamma-dependent CXCL10 is critical for accumulation of T cells and trypanosomes in the brain during experimental African trypanosomiasis. Data suggest CXCL10 as a candidate marker for late stage human African trypanosomiasis. en
dc.language English en
dc.subject Protozoal diseases en
dc.subject Trypanosomiasis, African en
dc.subject Encephalitic stage en
dc.subject Markers en
dc.subject Cytokines en
dc.subject Expression en
dc.subject CXCL10 en
dc.title Expression and role of CXCL10 during the encephalitic stage of experimental and clinical African trypanosomiasis en
dc.type Article en
dc.citation.issue 10 en
dc.citation.jtitle Journal of Infectious Diseases en
dc.citation.volume 200 en
dc.citation.pages 1556-1565 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/19827943
dc.citation.jabbreviation J Infect Dis en


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