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Natural infection of Phlebotomus argentipes with Leishmania and other trypanosomatids in a visceral leishmaniasis endemic region of Nepal

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dc.contributor.author Bhattarai, N. R.
dc.contributor.author Das, M. L.
dc.contributor.author Rijal, S.
dc.contributor.author Van der Auwera, G.
dc.contributor.author Picado, A.
dc.contributor.author Khanal, B.
dc.contributor.author Roy, L.
dc.contributor.author Speybroeck, N.
dc.contributor.author Berkvens, D.
dc.contributor.author Davies, C. R.
dc.contributor.author Coosemans, M.
dc.contributor.author Boelaert, M.
dc.contributor.author Dujardin, J. C.
dc.date.accessioned 2009-12-18T12:42:28Z
dc.date.available 2009-12-18T12:42:28Z
dc.date.issued 2009
dc.identifier.issn 0035-9203
dc.identifier.doi http://dx.doi.org/10.1016/j.trstmh.2009.03.008
dc.identifier.other ITG-P1B
dc.identifier.other ITG-P4A
dc.identifier.other ITG-A8A
dc.identifier.other ITG-A9A
dc.identifier.other ITG-P11A
dc.identifier.other ITG-H12A
dc.identifier.other ITG-PLA
dc.identifier.other MULTI-3
dc.identifier.other PARAS
dc.identifier.other U-PROTO
dc.identifier.other U-ENTOM
dc.identifier.other ANIMAL
dc.identifier.other U-ANIMAL
dc.identifier.other HEALTH
dc.identifier.other U-EPID
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other UPD17
dc.identifier.other ABSTRACT
dc.identifier.uri http://hdl.handle.net/10390/2846
dc.description.abstract Monitoring Leishmania infection in sand flies is important for understanding the eco-epidemiology of kala-azar and assessing the impact of the recently launched kala-azar control programme in the Indian subcontinent. We applied a PCR technique that targets rRNA genes to estimate the natural incidence of Leishmania infection in sand flies sampled in six villages of the Terai region of Nepal. Amplifications were made on 135 pools of sand flies and confirmed by sequencing. Seven pools were found to be PCR positive: in five of them we identified the rDNA signature found in Leishmania spp., whereas two other pools revealed a sequence compatible with other trypanosomatids. Different methodologies were applied to evaluate the infection rate from pools of unequal size and estimated the infection rate to range from 0.468% to 0.578% for the Leishmania group and from 0.185% to 0.279% for the non-Leishmania group. Our results highlight the diversity of flagellate infections likely to be encountered in Phlebotomus argentipes populations. Our methodology allows clear discrimination of Leishmania from other trypanosomatids and should be applied on larger insect samples or in longitudinal studies. en
dc.language English en
dc.subject Protozoal diseases en
dc.subject Leishmaniasis en
dc.subject Visceral en
dc.subject Kala azar en
dc.subject Eco-epidemiology en
dc.subject Sand flies en
dc.subject Phlebotomus argentipes en
dc.subject Incidence en
dc.subject Infection rates en
dc.subject PCR en
dc.subject Ribosomal DNA en
dc.subject Nepal en
dc.subject Asia, South en
dc.title Natural infection of Phlebotomus argentipes with Leishmania and other trypanosomatids in a visceral leishmaniasis endemic region of Nepal en
dc.type Article en
dc.citation.issue 11 en
dc.citation.jtitle Transactions of the Royal Society of Tropical Medicine and Hygiene en
dc.citation.volume 103 en
dc.citation.pages 1087-1092 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/19345387
dc.citation.jabbreviation Trans R Soc Trop Med Hyg en


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