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Freezing of stool samples improves real-time PCR detection of Entamoeba dispar and Entamoeba histolytica

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Freezing of stool samples improves real-time PCR detection of Entamoeba dispar and Entamoeba histolytica

Cnops, L.; Van Esbroeck, M.
Journal Name:
Journal of Microbiological Methods
Issue Date:
2010
Volume:
80
Issue:
3
Pages:
310-312
DOI:
http://dx.doi.org/10.1016/j.mimet.2010.01.001
Pubmed ID:
http://www.ncbi.nlm.nih.gov/pubmed/20085792
Language:
English
Type:
Article
Keywords:
Protozoal diseases
Amebiasis
Entamoeba histolytica
Entamoeba dispar
Laboratory diagnosis
Differential diagnosis
Serology
Antigen detection
DNA
Real-time
PCR
Stool examination

Abstract:

PCR as a tool for intestinal parasite diagnosis is expanding since differentiation between Entamoeba histolytica and Entamoeba dispar cysts is impossible with microscopy. Since pre-analytical factors influence DNA detection, we evaluated with real-time PCR the influence of storage time and temperature. We demonstrated an improved DNA detection in frozen stool samples. Copyright 2010 Elsevier B.V. All rights reserved.

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