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Phylogeny of Leishmania species based on the heat-shock protein 70 gene

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dc.contributor.author Fraga, J.
dc.contributor.author Montalvo, A. M.
dc.contributor.author De Doncker, S.
dc.contributor.author Dujardin, J. C.
dc.contributor.author Van der Auwera, G.
dc.date.accessioned 2010-03-17T13:20:33Z
dc.date.available 2010-03-17T13:20:33Z
dc.date.issued 2010
dc.identifier.issn 1567-1348
dc.identifier.doi http://dx.doi.org/10.1016/j.meegid.2009.11.007
dc.identifier.other ITG-P3B
dc.identifier.other ITG-P4A
dc.identifier.other ITG-PLB
dc.identifier.other PARAS
dc.identifier.other U-PROTO
dc.identifier.other UPD19
dc.identifier.other ABSTRACT
dc.identifier.uri http://hdl.handle.net/10390/3001
dc.description.abstract The 70kDa heat-shock protein (HSP70) is conserved across prokaryotes and eukaryotes, and the protein as well as its encoding gene have been applied in phylogenetic studies of different parasites. In spite of the frequent use of New World Leishmania species identification on the basis of restriction fragment length polymorphisms (RFLP) in the hsp70 gene, it was never sequenced extensively for studying evolutionary relationships. To fill this void we determined the nucleotide sequence of an 1380bp fragment of the coding region commonly used in RFLP analysis, from 43 isolates and strains of different geographic origins. Combination with previously determined sequences amounted to a phylogenetic analysis including 52 hsp70 sequences representing 17 species commonly causing leishmaniasis both in the New and Old World. The genus Leishmania formed a monophyletic group with three distinct subgenera L. (Leishmania), L. (Viannia), and L. (Sauroleishmania). The obtained phylogeny supports the following eight species: L. (L.) donovani, L. (L.) major, L. (L.) tropica, L. (L.) mexicana, L. (V.) lainsoni, L. (V.) naiffi, L. (V.) guyanensis and L. (V.) braziliensis, in some of which subspecies can be recognized: L. (L.) donovani infantum, L. (V.) guyanensis panamensis, and L. (V.) braziliensis peruviana. The currently recognized L. (L.) aethiopica, L. (L.) garnhami, and L. (L.) amazonensis did not form monophyletic clusters. These findings are discussed in relation to results from other genes and proteins, which have to be integrated in order to build a genetically supported taxonomy for the entire genus. en
dc.language English en
dc.subject Protozoal diseases en
dc.subject Leishmaniasis en
dc.subject Leishmania donovani en
dc.subject Leishmania major en
dc.subject Leishmania mexicana en
dc.subject Leishmania lainsoni en
dc.subject Leishmania naiffi en
dc.subject Leishmania guyanensis en
dc.subject Leishmania braziliensis en
dc.subject Vectors en
dc.subject Sandflies en
dc.subject Phlebotomus argentipes en
dc.subject Taxonomy en
dc.subject Phylogeny en
dc.subject Heat-shock proteins en
dc.subject Nucleotide sequences en
dc.subject Primers en
dc.subject Clusters en
dc.title Phylogeny of Leishmania species based on the heat-shock protein 70 gene en
dc.type Article en
dc.citation.issue 2 en
dc.citation.jtitle Infection, Genetics and Evolution en
dc.citation.volume 10 en
dc.citation.pages 238-245 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/19913110
dc.citation.jabbreviation Infect Genet Evol en


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