Institute of Tropical Medicine Antwerp
Foundation of Public Utility

Trypanosoma cruzi: variability of stocks from Colombia determined by molecular karyotype and minicircle Southern blot analysis

DSpace/Manakin Repository

Show simple item record

dc.contributor.author Triana, O. en_US
dc.contributor.author Ortiz, S. en_US
dc.contributor.author Dujardin, J. C. en_US
dc.contributor.author Solari, A. en_US
dc.date.accessioned 2007-12-06T14:34:18Z
dc.date.available 2007-12-06T14:34:18Z
dc.date.issued 2006 en_US
dc.identifier.issn 0014-4894 en_US
dc.identifier.doi http://dx.doi.org/10.1016/j.exppara.2005.11.016
dc.identifier.other ITG-P3A en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-PROTO en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/306
dc.description.abstract Nineteen Trypanosoma cruzi stocks, most of them of wild origin, and four Trypanosoma rangeli stocks from Colombia were analysed by molecular karyotype analysis with cloned DNA cruzipain as the probe. Another 27 cloned stocks of T. cruzi from different geographic areas of South America were used as reference for T. cruzi lineages. Phenetic analysis of chromosome size polymorphism demonstrated a great variability of Colombian T. cruzi stocks, suggesting that most belong to lineage I, although two of them belong to lineage II. The 2 lineage II T. cruzi, 17 T. cruzi lineage I, and 3 T. rangeli stocks from Colombia were studied further by Southern blot analysis with a panel of kinetoplast DNA minicircle probes. Hybridisation results indicate that the two T. cruzi II stocks are genetically distant from each other and from T. cruzi lineages IIb, IId, and IIe from Chile. Finally, T. cruzi minicircle probes do not cross-hybridise in any stringency condition tested with T. rangeli minicircles, a clear indication that these parasites can be easily distinguished by this method. en_US
dc.language English en_US
dc.subject Protozoology en_US
dc.subject Trypanosoma cruzi en_US
dc.subject Trypanosoma rangeli en_US
dc.subject Phylogenetics en_US
dc.subject Genetic variation en_US
dc.subject Karyotypes en_US
dc.subject Colombia en_US
dc.subject America, Latin en_US
dc.title Trypanosoma cruzi: variability of stocks from Colombia determined by molecular karyotype and minicircle Southern blot analysis en_US
dc.type Article en_US
dc.citation.issue 1 en_US
dc.citation.jtitle Experimental Parasitology en_US
dc.citation.volume 113 en_US
dc.citation.pages 62-66 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/16388803
dc.citation.jabbreviation Exp Parasitol en_US


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record