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Presence of Anopheles culicifacies B in Cambodia established by the PCR-RFLP assay developed for the identification of Anopheles minimus species A and C and four related species

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dc.contributor.author Van Bortel, W. en_US
dc.contributor.author Sochanta, T. en_US
dc.contributor.author Harbach, R. E. en_US
dc.contributor.author Socheat, D. en_US
dc.contributor.author Roelants, P. en_US
dc.contributor.author Backeljau, T. en_US
dc.contributor.author Coosemans, M. en_US
dc.date.accessioned 2007-12-06T14:34:23Z
dc.date.available 2007-12-06T14:34:23Z
dc.date.issued 2002 en_US
dc.identifier.issn 0269-283X en_US
dc.identifier.doi http://dx.doi.org/10.1046/j.1365-2915.2002.00376.x
dc.identifier.other ITG-P1A en_US
dc.identifier.other ITG-P5B en_US
dc.identifier.other ITG-PLA en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-ENTOM en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/321
dc.description.abstract A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay developed for identification of five species of the Anopheles minimus Theobald group and a related mosquito species of the Myzomyia Series (Diptera: Culicidae) was applied to morphologically identified adult female specimens collected in Ratanakiri Province, north-eastern Cambodia. In addition to finding An. aconitus Dönitz, An. minimus species A and An. pampanai Büttiker & Beales, some specimens showed a new restriction banding pattern. Siblings of specimens that exhibited this new PCR-RFLP pattern were morphologically identified as An. culicifacies James sensu lato. Based on nucleotide sequences of the ribonuclear DNA internal transcribed spacer 2 region (ITS2) and the mitochondrial cytochrome oxidase I gene (COI), these specimens were recognized as An. culicifacies species B (sensu Green & Miles, 1980), the first confirmed record of the An. culicifacies complex from Cambodia. This study shows that the PCR-RFLP assay can detect species not included in the initial set-up and is capable of identifying at least seven species of the Myzomyia Series, allowing better definition of those malaria vector and non-vector anophelines in South-east Asia. en_US
dc.language English en_US
dc.subject Protozoal diseases en_US
dc.subject Malaria en_US
dc.subject Vectors en_US
dc.subject Entomology en_US
dc.subject Mosquitoes en_US
dc.subject Species en_US
dc.subject Anopheles culicifacies en_US
dc.subject Anopheles minimus en_US
dc.subject Identification en_US
dc.subject Assays en_US
dc.subject PCR-RFLP en_US
dc.subject Cambodia en_US
dc.subject Asia, Southeast en_US
dc.title Presence of Anopheles culicifacies B in Cambodia established by the PCR-RFLP assay developed for the identification of Anopheles minimus species A and C and four related species en_US
dc.type Article en_US
dc.citation.issue 3 en_US
dc.citation.jtitle Medical and Veterinary Entomology en_US
dc.citation.volume 16 en_US
dc.citation.pages 329-334 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/12243235
dc.citation.jabbreviation Med Vet Entomol en_US


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