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Molecular identification of Anopheles minimus s.l. beyond distinguishing the members of the species complex

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dc.contributor.author Van Bortel, W. en_US
dc.contributor.author Trung, H. D. en_US
dc.contributor.author Roelants, P. en_US
dc.contributor.author Harbach, R. E. en_US
dc.contributor.author Backeljau, T. en_US
dc.contributor.author Coosemans, M. en_US
dc.date.accessioned 2007-12-06T14:34:23Z
dc.date.available 2007-12-06T14:34:23Z
dc.date.issued 2000 en_US
dc.identifier.other ITG-P1A en_US
dc.identifier.other ITG-P3B en_US
dc.identifier.other ITG-PLA en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-ENTOM en_US
dc.identifier.other JIF en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/323
dc.description.abstract Correct species identification is the starting point for studying the epidemiological role of vectors. Identification is mostly achieved using morphological criteria, but this fails when sibling species and species with overlapping morphological characters are involved. The problem with the identification of Anopheles minimus s. l., one of the most widespread malaria vectors in South-East Asia, is twofold: it is a complex of at least two isomorphic species, and based on morphology, members of the complex are difficult to distinguish from closely related species. An identification method was developed for An. minimus species A and C, and four related species, An. aconitus, An. pampanai, An. varuna and An. jeyporiensis. PCR-amplified internal transcribed spacer 2 (ITS2) ribonuclear DNA (rDNA) fragments were digested with restriction endonuclease BsiZI. Clear diagnostic banding patterns for the six species were obtained on agarose gels. Testing field-collected specimens from different regions in South-East Asia indicated that the technique will be applicable over a wide geographical area. From this it is clear that molecular identification has to focus not only on the species of complexes, but also on related species if they hamper the morphological identification of the 'sensu lato species'. en_US
dc.language English en_US
dc.subject Entomology en_US
dc.subject Anopheles minimus en_US
dc.subject Identification en_US
dc.subject Molecular biology en_US
dc.title Molecular identification of Anopheles minimus s.l. beyond distinguishing the members of the species complex en_US
dc.type Article en_US
dc.citation.jtitle Insect Molecular Biology en_US
dc.citation.volume 9 en_US
dc.citation.pages 335-340 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/10886418
dc.citation.jabbreviation Insect Mol Biol en_US


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