Institute of Tropical Medicine Antwerp
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The Glossina morsitans tsetse fly saliva: general characteristics and identification of novel salivary proteins

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Show simple item record Van Den Abbeele, J. en_US Caljon, G. en_US Dierick, J. F. en_US Moens, L. en_US De Ridder, K. en_US Coosemans, M. en_US 2007-12-06T14:34:24Z 2007-12-06T14:34:24Z 2007 en_US
dc.identifier.issn 0965-1748 en_US
dc.identifier.other ITG-P1A en_US
dc.identifier.other ITG-PLA en_US
dc.identifier.other ITG-P5B en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-ENTOM en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.description.abstract The tsetse fly (Glossina spp.) is an obligate blood-sucking insect that transmits different human-pathogenic and livestock threatening trypanosome species in Africa. To obtain more insight in the tsetse salivary function, some general aspects of the tsetse fly saliva and its composition were studied. Direct pH and protein content measurements revealed a moderately alkaline (pH approximately 8.0) salivary environment with approximately 4.3 microg soluble proteins per gland and a constant representation of the major saliva proteins throughout the blood-feeding cycle. Although major salivary genes are constitutively expressed, upregulation of salivary protein synthesis within 48 h after the blood meal ensures complete protein replenishment from day 3 onwards. Screening of a non-normalised Glossina morsitans morsitans lambdagt11 salivary gland expression library with serum from a saliva-immunized rabbit identified three full-length cDNAs encoding for novel salivary proteins with yet unknown functions: a 8.3 kDa glycine/glutamate-rich protein (G. morsitans morsitans salivary gland protein Gmmsgp1), a 12.0 kDa proline-rich protein (Gmmsgp2), and a 97.4 kDa protein composed of a metallophosphoesterase/5'nucleotidase region with a glutamate/aspartate/asparagines-rich region (Gmmsgp3). en_US
dc.language English en_US
dc.subject Entomology en_US
dc.subject Tsetse flies en_US
dc.subject Glossina morsitans en_US
dc.subject Saliva en_US
dc.subject Proteins en_US
dc.title The Glossina morsitans tsetse fly saliva: general characteristics and identification of novel salivary proteins en_US
dc.type Article en_US
dc.citation.issue 10 en_US
dc.citation.jtitle Insect Biochemistry and Molecular Biology en_US
dc.citation.volume 37 en_US
dc.citation.pages 1075-1085 en_US
dc.citation.jabbreviation Insect Biochem Mol Biol en_US

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