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General expression of RoTat 1.2 variable antigen type in Trypanosoma evansi isolates from different origin

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dc.contributor.author Verloo, D. en_US
dc.contributor.author Magnus, E. en_US
dc.contributor.author Büscher, P. en_US
dc.date.accessioned 2007-12-06T14:34:31Z
dc.date.available 2007-12-06T14:34:31Z
dc.date.issued 2001 en_US
dc.identifier.issn 0304-4017 en_US
dc.identifier.doi http://dx.doi.org/10.1016/S0304-4017(01)00412-5
dc.identifier.other ITG-P1B en_US
dc.identifier.other ITG-P2B en_US
dc.identifier.other ITG-PLA en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-SEROL en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/347
dc.description.abstract The variable surface glycoprotein of Trypanosoma evansi RoTat 1.2 variable antigen type (VAT) is used as an antigen in different antibody detection assays for T. evansi. To obtain more information on the predominant character of RoTat 1.2 and its diagnostic potential in antibody detection tests, we checked its expression in 10 different T. evansi stocks and clones from different parts of the world. Cryostabilates were injected into mice and the trypanosomes of the first peak parasitaemia were screened for the presence of RoTat 1.2 by VAT specific immunofluorescence. To monitor the appearance of RoTat 1.2 specific antibodies during infection, rabbits were infected and serologically tested at different time intervals with VAT specific immune trypanolysis, CATT/T. evansi, LATEX/T. evansi and ELISA/T. evansi.Test results confirm the predominant character of RoTat 1.2. en_US
dc.language English en_US
dc.publisher Elsevier
dc.subject Protozoal diseases en_US
dc.subject Trypanosoma evansi en_US
dc.subject Laboratory diagnosis en_US
dc.subject Antigens, differentiation en_US
dc.subject Laboratory techniques and procedures en_US
dc.title General expression of RoTat 1.2 variable antigen type in Trypanosoma evansi isolates from different origin en_US
dc.type Article en_US
dc.citation.issue 3 en_US
dc.citation.jtitle Veterinary Parasitology en_US
dc.citation.volume 97 en_US
dc.citation.pages 183-189 en_US
dc.publisher.place Amsterdam
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/11390070
dc.identifier.url http://www.elsevier.com/locate/vetpar
dc.citation.jabbreviation Vet Parasitol en_US


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