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Characterization of subunits of the RNA polymerase I complex in Trypanosoma brucei

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Show simple item record Walgraffe, D. en_US Devaux, S. en_US Lecordier, L. en_US Dierick, J. F. en_US Dieu, M. en_US Van den Abbeele, J. en_US Pays, E. en_US Vanhamme, L. en_US 2007-12-06T14:34:34Z 2007-12-06T14:34:34Z 2005 en_US
dc.identifier.issn 0166-6851 en_US
dc.identifier.other ITG-P6A en_US
dc.identifier.other PARAS en_US
dc.identifier.other U-ENTOM en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.description.abstract The Trypanosoma brucei homologue of the RNA polymerase I (RNA Pol I) subunit Rpa12p of Saccharomyces cerevisiae was cloned and characterized. This protein did not appear to be essential for growth in either bloodstream or procyclic forms of the parasite. Trypanosomes expressing a C-terminal tagged version of TbRPA12 were generated in order to purify RNA Pol I from both developmental stages. Tandem affinity purification (TAP) revealed a number of proteins associating with TbRPA12, some of which appeared to be stage-specific. Mass spectrometry allowed the identification of four subunits in addition to TbRPA12, namely TbRPA1, TbRPA2, TbRPC40 and one isoform of TbRPB5 (Tb1RPB5), as well as an unknown 30kDa protein and histones H2A and H3. Whereas these studies demonstrated that TbRPA1 was phosphorylated, no evidence for phosphorylation of TbRPA2 was found. en_US
dc.language English en_US
dc.publisher Elsevier
dc.subject Protozoology en_US
dc.subject Trypanosoma brucei en_US
dc.subject Characterization en_US
dc.subject Antigenic variation en_US
dc.subject Variant surface glycoproteins en_US
dc.subject Expression sites en_US
dc.subject Transcription en_US
dc.subject Polycistronic en_US
dc.title Characterization of subunits of the RNA polymerase I complex in Trypanosoma brucei en_US
dc.type Article en_US
dc.citation.issue 2 en_US
dc.citation.jtitle Molecular and Biochemical Parasitology en_US
dc.citation.volume 139 en_US
dc.citation.pages 249-260 en_US Amsterdam
dc.citation.jabbreviation Mol Biochem Parasitol en_US

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