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Isolation of a 14 kDa antigen from Taenia solium cyst fluid by HPLC and its evaluation in enzyme linked immunosorbent assay for diagnosis of porcine cysticercosis

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dc.contributor.author Assana, E. en_US
dc.contributor.author Kanobana, K. en_US
dc.contributor.author Tume, C. B. en_US
dc.contributor.author Zoli, P. A. en_US
dc.contributor.author Nguekam en_US
dc.contributor.author Geerts, S. en_US
dc.contributor.author Berkvens, D. en_US
dc.contributor.author Dorny, P. en_US
dc.date.accessioned 2007-12-06T14:35:40Z
dc.date.available 2007-12-06T14:35:40Z
dc.date.issued 2007 en_US
dc.identifier.issn 0034-5288 en_US
dc.identifier.doi http://dx.doi.org/10.1016/j.rvsc.2006.09.006
dc.identifier.other ITG-A1B en_US
dc.identifier.other ITG-A2B en_US
dc.identifier.other ITG-A6A en_US
dc.identifier.other ITG-A7A en_US
dc.identifier.other ITG-ALA en_US
dc.identifier.other ANIMAL en_US
dc.identifier.other U-ANIMAL en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/410
dc.description.abstract A fraction with a major band of 14kDa was obtained from crude cyst fluid of Taenia solium cysticerci by 2-step chromatography. A first fraction isolated by gel filtration (Sephacryl S-300 high resolution) was purified using an anion exchange column (Mono Q HR 5/5) on high performance liquid chromatography. Evaluation of the analytic sensitivity of this fraction (F3) was carried out in an antibody enzyme linked immunosorbent assay (Ab-ELISA-F3) using serum samples from pigs experimentally infected with different doses of T. solium eggs. The cross-reactivity of F3 was evaluated with serum samples from pigs that were naturally or experimentally infected with Taenia hydatigena, Taenia saginata asiatica, Fasciola hepatica, Trichinella spiralis, Metastrongylus apri, Trypanosoma congolense and Sarcoptes scabiei, and with serum samples of rabbits hyper-immunised with metacestode cyst fluid of T. hydatigena and T. solium. Antibody titres of lightly or heavily infected pigs differed in their kinetics. However, the increase in F3-specific antibodies could not be related to the infection level. Analysis of the specificity of the F3 showed that serum samples of pigs infected with other parasites did not recognise this antigen. Cross-reaction with T. hydatigena occurred in ELISA using cyst fluid as antigen, but the F3 antigen fraction was not recognized by rabbit hyper-immune serum samples to T. hydatigena. Evaluation of the diagnostic sensitivity and specificity of the Ab-ELISA-F3 was done by a non-parametric receiver operating characteristic (ROC) analysis using 66 serum samples from Zambian village pigs. The total number of cysticerci of these pigs was determined by dissection (28 pigs harboured T. solium cysticerci and 38 were negative at dissection). In addition, 58 serum samples from Cameroonian pigs (28 pigs from cysticercosis-free farms and 30 pigs with cysticerci at tongue inspection) were used in a separate ROC analysis. The results from the ROC analysis yielded a low diagnostic value (area under ROC curve=0.48) with the sera from the Zambian pigs while a relatively high diagnostic value was obtained with the sera from Cameroonian pigs (area under ROC curve=0.78). The main factor contributing to a low diagnostic value based on the Zambian serum samples seemed to be the false-positive reactions that were likely caused by the occurrence of transient antibodies in the non-infected animals. en_US
dc.language English en_US
dc.subject Helminthic diseases en_US
dc.subject Cysticercosis en_US
dc.subject Taenia solium en_US
dc.subject Laboratory diagnosis en_US
dc.subject Cyst fluid en_US
dc.subject Purification en_US
dc.subject ELISA en_US
dc.subject ROC en_US
dc.subject Sensitivity en_US
dc.subject Specificity en_US
dc.subject Laboratory techniques and procedures en_US
dc.title Isolation of a 14 kDa antigen from Taenia solium cyst fluid by HPLC and its evaluation in enzyme linked immunosorbent assay for diagnosis of porcine cysticercosis en_US
dc.type Article en_US
dc.citation.issue 3 en_US
dc.citation.jtitle Research in Veterinary Science en_US
dc.citation.volume 82 en_US
dc.citation.pages 370-376 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/17101161
dc.citation.jabbreviation Res Vet Sci en_US


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