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Reactive oxygen species and 12/15-lipoxygenase contribute to the antiproliferative capacity of alternatively activated myeloid cells elicited during helminth infection

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dc.contributor.author Brys, L. en_US
dc.contributor.author Beschin, A. en_US
dc.contributor.author Raes, G. en_US
dc.contributor.author Ghassabeh, G. H. en_US
dc.contributor.author Noël, W. en_US
dc.contributor.author Brandt, J. en_US
dc.contributor.author Brombacher, F. en_US
dc.contributor.author De Baetselier, P. en_US
dc.date.accessioned 2007-12-06T14:35:49Z
dc.date.available 2007-12-06T14:35:49Z
dc.date.issued 2005 en_US
dc.identifier.issn 0022-1767 en_US
dc.identifier.other ITG-A6A en_US
dc.identifier.other ANIMAL en_US
dc.identifier.other U-ANIMAL en_US
dc.identifier.other JIF en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/436
dc.description.abstract Understanding the role of CD11b(+)GR-1(+) myeloid suppressor cells in the immune suppression and immunoregulation associated with a variety of diseases may provide therapeutic opportunities. In this article, we show, in a model of helminth infection, that CD11b(+)GR-1(+) myeloid suppressor cells but not CD11b(+)F4/80(high) mature macrophages expanded in the peritoneal cavity of BALB/c mice implanted with Taenia crassiceps. Peritoneal cell populations from early stage-infected animals impaired T cell proliferation by secreting NO. Yet, they lost their ability to secrete NO in the late stage of infection. Concomitantly, their capacity to exert arginase activity and to express mRNAs coding for FIZZ1 (found in inflammatory zone 1), Ym, and macrophage galactose-type C-type lectin increased. Furthermore, cells from early stage-infected mice triggered T cells to secrete IFN-gamma and IL-4, whereas in the late stage of infection, they only induced IL-4 production. These data suggest that CD11b(+)GR-1(+) myeloid suppressor cells displaying an alternative activation phenotype emerged gradually as T. crassiceps infection progressed. Corroborating the alternative activation status in the late stage of infection, the suppressive activity relied on arginase activity, which facilitated the production of reactive oxygen species including H(2)O(2) and superoxide. We also document that the suppressive activity of alternative myeloid suppressor cells depended on 12/15-lipoxygenase activation generating lipid mediators, which triggered peroxisome proliferator-activated receptor-gamma. IL-4 and IL-13 signaling contributed to the expansion of myeloid suppressor cells in the peritoneal cavity of T. crassiceps-infected animals and to their antiproliferative activity by allowing arginase and 12/15-lipoxygenase gene expression. en_US
dc.language English en_US
dc.subject Helminthic diseases en_US
dc.subject Taenia crassiceps en_US
dc.subject Mice en_US
dc.subject Immune response en_US
dc.subject Myeloid cells en_US
dc.subject T-lymphocytes, suppressor-effector en_US
dc.title Reactive oxygen species and 12/15-lipoxygenase contribute to the antiproliferative capacity of alternatively activated myeloid cells elicited during helminth infection en_US
dc.type Article en_US
dc.citation.jtitle Journal of Immunology en_US
dc.citation.volume 174 en_US
dc.citation.pages 6095-6104 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/15879104
dc.citation.jabbreviation J Immunol en_US


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