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PCR-RFLP using Ssu-rDNA amplification as an easy method for species-specific diagnosis of Trypanosoma species in cattle

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dc.contributor.author Geysen, D. en_US
dc.contributor.author Delespaux, V. en_US
dc.contributor.author Geerts, S. en_US
dc.date.accessioned 2007-12-06T14:36:14Z
dc.date.available 2007-12-06T14:36:14Z
dc.date.issued 2003 en_US
dc.identifier.issn 0304-4017 en_US
dc.identifier.doi http://dx.doi.org/10.1016/S0304-4017(02)00313-8
dc.identifier.other ITG-A1A en_US
dc.identifier.other ITG-A2A en_US
dc.identifier.other ITG-ALA en_US
dc.identifier.other ANIMAL en_US
dc.identifier.other U-ANIMAL en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/520
dc.description.abstract A single polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) assay was used to characterise all important bovine trypanosome species. This is the first report of a sensitive pan-trypanosome PCR assay amplifying all species including T. vivax to a comparable extent using a single primer pair. A semi-nested PCR approach resulted in the detection of one T. congolense trypanosome genome/40 microl of blood, applied as buffy coat on filter paper. Restriction enzyme analysis using Msp1 and Eco571 gave a clear distinction between T. congolense, T. brucei, T. vivax and T. theileri. Several subgroups within the T. congolense group could be distinguished but no differences between the species belonging to the subgenus Trypanozoon or between T. simiae and T. theileri could be found. The use of MboII restriction enzyme allowed differentiation between T. simiae and T. theileri. The potential of the essay to be used as a suitable diagnostic tool is discussed. en_US
dc.language English en_US
dc.publisher Elsevier
dc.subject Animal diseases en_US
dc.subject Cattle en_US
dc.subject Protozoal diseases en_US
dc.subject Trypanosomiasis en_US
dc.subject Trypanosoma en_US
dc.subject Diagnosis en_US
dc.subject Identification en_US
dc.subject Tests en_US
dc.title PCR-RFLP using Ssu-rDNA amplification as an easy method for species-specific diagnosis of Trypanosoma species in cattle en_US
dc.type Article en_US
dc.citation.issue 3-4 en_US
dc.citation.jtitle Veterinary Parasitology en_US
dc.citation.volume 110 en_US
dc.citation.pages 171-180 en_US
dc.publisher.place Amsterdam
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/12482646
dc.identifier.url http://www.elsevier.com/locate/vetpar
dc.citation.jabbreviation Vet Parasitol en_US


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