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A modified AFLP for Trypanosoma congolense isolate characterisation

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dc.contributor.author Masumu, J. en_US
dc.contributor.author Geysen, D. en_US
dc.contributor.author Vansnick, E. en_US
dc.contributor.author Geerts, S. en_US
dc.contributor.author Van den Bossche, P. en_US
dc.date.accessioned 2007-12-06T14:36:29Z
dc.date.available 2007-12-06T14:36:29Z
dc.date.issued 2006 en_US
dc.identifier.issn 0168-1656 en_US
dc.identifier.doi http://dx.doi.org/10.1016/j.jbiotec.2006.01.016
dc.identifier.other ITG-A1B en_US
dc.identifier.other ITG-A2A en_US
dc.identifier.other ITG-A3B en_US
dc.identifier.other ITG-A4A en_US
dc.identifier.other ITG-ALA en_US
dc.identifier.other ANIMAL en_US
dc.identifier.other U-ANIMAL en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/568
dc.description.abstract The amplified fragment length polymorphism (AFLP) technique is a reliable and powerful DNA fingerprint tool for genetic characterisation and analysis. In this paper, we described a modified AFLP with high resolution for Trypanosoma congolense using one enzyme and agarose or Elchrom gel electrophoresis. Eleven allopatric and fourteen sympatric isolates of T. congolense savannah were used to assess the resolution of the method and its ability to characterise T. congolense isolates. Two enzymes (Eco RI or Bgl II) and corresponding non-selective and selective primers were used to identify the most appropriate combination. Patterns generated by Bgl II enzyme and a single selective primer A, C, G or T produced clear profiles. Each of the four selective primers produced different profiles for all the 25 T. congolense isolates. Due to the reduction in the number of bands, profiles could be analysed using agarose or Elchrom gels. Although comparison of a great number of samples could benefit from software help, this technique did not require flurochrome detection methods. The results of the present study demonstrated that this modified AFLP makes the characterisation of T. congolense easier while maintaining high resolution. en_US
dc.language English en_US
dc.subject Protozoology en_US
dc.subject Trypanosoma congolense en_US
dc.subject Characterization en_US
dc.subject Genetic diversity en_US
dc.subject AFLP en_US
dc.subject Fingerprinting en_US
dc.subject Laboratory techniques and procedures en_US
dc.title A modified AFLP for Trypanosoma congolense isolate characterisation en_US
dc.type Article en_US
dc.citation.issue 1 en_US
dc.citation.jtitle Journal of Biotechnology en_US
dc.citation.volume 125 en_US
dc.citation.pages 22-26 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/16516323
dc.citation.jabbreviation J Biotechnol en_US


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