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New Trypanosoma (Duttonella) vivax genotypes from tsetse flies in East Africa

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dc.contributor.author Adams, E. R.
dc.contributor.author Hamilton, P. B.
dc.contributor.author Rodrigues, A. C.
dc.contributor.author Malele, I. I.
dc.contributor.author Delespaux, V.
dc.contributor.author Teixeira, M. M. G.
dc.contributor.author Gibson, W.
dc.date.accessioned 2010-04-21T09:00:31Z
dc.date.available 2010-04-21T09:00:31Z
dc.date.issued 2010
dc.identifier.issn 0031-1820
dc.identifier.doi http://dx.doi.org/10.1017/S0031182009991508
dc.identifier.other ITG-A5A
dc.identifier.other ANIMAL
dc.identifier.other U-ANIMAL
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other UPD20
dc.identifier.other ABSTRACT
dc.identifier.other FTA
dc.identifier.uri http://hdl.handle.net/10390/6104
dc.description.abstract Salivarian trypanosomes pose a substantial threat to livestock, but their full diversity is not known. To survey trypanosomes carried by tsetse in Tanzania, DNA samples from infected proboscides of Glossina pallidipes and G. swynnertoni were identified using fluorescent fragment length barcoding (FFLB), which discriminates species by size polymorphisms in multiple regions of the ribosomal RNA locus. FFLB identified the trypanosomes in 65 of 105 (61.9%) infected proboscides, revealing 9 mixed infections. Of 7 different FFLB profiles, 2 were similar but not identical to reference West African Trypanosoma vivax; 5 other profiles belonged to known species also identified in fly midguts. Phylogenetic analysis of the glycosomal glyceraldehyde phosphate dehydrogenase gene revealed that the Tanzanian T. vivax samples fell into 2 distinct groups, both outside the main clade of African and South American T. vivax. These new T. vivax genotypes were common and widespread in tsetse in Tanzania. The T. brucei-like trypanosome previously described from tsetse midguts was also found in 2 proboscides, demonstrating a salivarian transmission route. Investigation of mammalian host range and pathogenicity will reveal the importance of these new trypanosomes for the epidemiology and control of animal trypanosomiasis in East Africa. en
dc.language English en
dc.subject Protozoal diseases en
dc.subject Nagana en
dc.subject Trypanosoma vivax en
dc.subject Vectors en
dc.subject Tsetse flies en
dc.subject Glossina pallidipes en
dc.subject Glossina swynnertoni en
dc.subject Epidemiology en
dc.subject Livestock en
dc.subject Genotypes en
dc.subject Identification en
dc.subject Fluorescent fragment length barcoding en
dc.subject FFLB en
dc.subject Phylogeny en
dc.subject Strains en
dc.subject Genome en
dc.subject Amplification en
dc.subject Gene sequencing en
dc.subject Africa, East en
dc.title New Trypanosoma (Duttonella) vivax genotypes from tsetse flies in East Africa en
dc.type Article en
dc.citation.issue 4 en
dc.citation.jtitle Parasitology en
dc.citation.volume 137 en
dc.citation.pages 641-650 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/19961657
dc.citation.jabbreviation Parasitology en


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