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Development and evaluation of different PCR-based typing methods for discrimination of Leishmania donovani isolates from Nepal

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dc.contributor.author Bhattarai, N. R.
dc.contributor.author Dujardin, J. C.
dc.contributor.author Rijal, S.
dc.contributor.author De Doncker, S.
dc.contributor.author Boelaert, M.
dc.contributor.author Van der Auwera, G.
dc.date.accessioned 2010-05-18T14:31:45Z
dc.date.available 2010-05-18T14:31:45Z
dc.date.issued 2010
dc.identifier.issn 0031-1820
dc.identifier.issn ABSTRACT
dc.identifier.doi http://dx.doi.org/10.1017/S0031182009991752
dc.identifier.other ITG-P1B
dc.identifier.other ITG-P2A
dc.identifier.other ITG-P4B
dc.identifier.other ITG-H5A
dc.identifier.other ITG-PLA
dc.identifier.other MULTI
dc.identifier.other PARAS
dc.identifier.other U-PROTO
dc.identifier.other HEALTH
dc.identifier.other U-EPID
dc.identifier.other DOI
dc.identifier.other JIF
dc.identifier.other UPD21
dc.identifier.other FTA
dc.identifier.uri http://hdl.handle.net/10390/6133
dc.description.abstract INTRODUCTION: Leishmania donovani, the causative agent of visceral leishmaniasis in the Indian subcontinent, has been reported to be genetically homogeneous. In order to support ongoing initiatives to eliminate the disease, highly discriminative tools are required for documenting the parasite population and dynamics. METHODS: Thirty-four clinical isolates of L. donovani from Nepal were analysed on the basis of size and restriction endonuclease polymorphisms of PCR amplicons from kinetoplast minicircle DNA, 5 nuclear microsatellites, and nuclear loci encoding glycoprotein 63, cysteine proteinase B, and hydrophilic acylated surface protein B. We present and validate a procedure allowing standardized analysis of kDNA fingerprint patterns. RESULTS: Our results show that parasites are best discriminated on the basis of kinetoplast minicircle DNA (14 genotypes) and 1 microsatellite defining 7 genotypes, while the remaining markers discriminated 2 groups or were monomorphic. Combination of all nuclear markers revealed 8 genotypes, while extension with kDNA data yielded 18 genotypes. CONCLUSION: We present tools that allow discrimination of closely related L. donovani strains circulating in the Terai region of Nepal. These can be used to study the micro-epidemiology of parasite populations, determine the geographical origin of infections, distinguish relapses from re-infection, and monitor the spread of particular variants. en
dc.language English en
dc.subject Protozoal diseases en
dc.subject Leishmaniasis en
dc.subject Visceral en
dc.subject Leishmania donovani en
dc.subject Vectors en
dc.subject Sand flies en
dc.subject Isolation en
dc.subject Laboratory techniques and procedures en
dc.subject Polymerase chain reaction en
dc.subject PCR en
dc.subject RFLP en
dc.subject Genotyping en
dc.subject Tools en
dc.subject Strains en
dc.subject Glycoproteins en
dc.subject Geographical distribution en
dc.subject Microsatellites en
dc.subject Epidemiology en
dc.subject Nepal en
dc.subject Asia, South en
dc.title Development and evaluation of different PCR-based typing methods for discrimination of Leishmania donovani isolates from Nepal en
dc.type Article en
dc.citation.issue 6 en
dc.citation.jtitle Parasitology en
dc.citation.volume 137 en
dc.citation.pages 947-957 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/20109247
dc.citation.jabbreviation Parasitology en


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