Institute of Tropical Medicine Antwerp
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Transcriptome analysis of monocyte-HIV interactions

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dc.contributor.author Van den Bergh, R.
dc.contributor.author Florence, E.
dc.contributor.author Vlieghe, E.
dc.contributor.author Boonefaes, T.
dc.contributor.author Grooten, J.
dc.contributor.author Houthuys, E.
dc.contributor.author Tran Thi, T. H.
dc.contributor.author Gali, Y.
dc.contributor.author De Baetselier, P.
dc.contributor.author Vanham, G.
dc.contributor.author Raes, G.
dc.date.accessioned 2010-08-05T12:20:14Z
dc.date.available 2010-08-05T12:20:14Z
dc.date.issued 2010
dc.identifier.issn 1742-4690
dc.identifier.doi http://dx.doi.org/10.1186/1742-4690-7-53
dc.identifier.other ITG-C2A
dc.identifier.other ITG-C3A
dc.identifier.other ITG-M8B
dc.identifier.other ITG-M10A
dc.identifier.other MULTI
dc.identifier.other CLINIC
dc.identifier.other U-HIVCLI
dc.identifier.other MICRO
dc.identifier.other U-VIROL
dc.identifier.other URL
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other Electronic
dc.identifier.other FTA
dc.identifier.other Abstract
dc.identifier.other UPD23
dc.identifier.uri http://hdl.handle.net/10390/6222
dc.description.abstract BACKGROUND: During HIV infection and/or antiretroviral therapy (ART), monocytes and macrophages exhibit a wide range of dysfunctions which contribute significantly to HIV pathogenesis and therapy-associated complications. Nevertheless, the molecular components which contribute to these dysfunctions remain elusive. We therefore applied a parallel approach of genome-wide microarray analysis and focused gene expression profiling on monocytes from patients in different stages of HIV infection and/or ART to further characterise these dysfunctions. RESULTS: Processes involved in apoptosis, cell cycle, lipid metabolism, proteasome function, protein trafficking and transcriptional regulation were identified as areas of monocyte dysfunction during HIV infection. Individual genes potentially contributing to these monocyte dysfunctions included several novel factors. One of these is the adipocytokine NAMPT/visfatin, which we show to be capable of inhibiting HIV at an early step in its life cycle. Roughly half of all genes identified were restored to control levels under ART, while the others represented a persistent dysregulation. Additionally, several candidate biomarkers (in particular CCL1 and CYP2C19) for the development of the abacavir hypersensitivity reaction were suggested. CONCLUSIONS: Previously described areas of monocyte dysfunction during HIV infection were confirmed, and novel themes were identified. Furthermore, individual genes associated with these dysfunctions and with ART-associated disorders were pinpointed. These genes form a useful basis for further functional studies concerning the contribution of monocytes/macrophages to HIV pathogenesis. One such gene, NAMPT/visfatin, represents a possible novel restriction factor for HIV en
dc.language English en
dc.subject Viral diseases en
dc.subject HIV en
dc.subject AIDS en
dc.subject Infection en
dc.subject Treatment en
dc.subject HAART en
dc.subject Antiretrovirals en
dc.subject Monocytes en
dc.subject In vitro en
dc.subject Macrophages en
dc.subject Gene expression en
dc.subject Apoptosis en
dc.subject Lipids en
dc.subject Metabolism en
dc.subject Proteins en
dc.subject ELISA en
dc.subject Western blot en
dc.subject Transcriptome analysis en
dc.title Transcriptome analysis of monocyte-HIV interactions en
dc.type Article-E en
dc.citation.issue 53 en
dc.citation.jtitle Retrovirology en
dc.citation.volume 7 en
dc.identifier.pmid http://whttp://www.ncbi.nlm.nih.gov/pubmed/20546557
dc.identifier.url ww.retrovirology.com/content/7/1/53
dc.citation.jabbreviation Retrovirology en


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