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Phase II evaluation of sensitivity and specificity of PCR and NASBA followed by oligochromatography for diagnosis of human African trypanosomiasis in clinical samples from D.R. Congo and Uganda

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dc.contributor.author Matovu, E.
dc.contributor.author Mugasa, C. M.
dc.contributor.author Ekangu, R. A.
dc.contributor.author Deborggraeve, S.
dc.contributor.author Lubega, G. W.
dc.contributor.author Laurent, T.
dc.contributor.author Schoone, G. J.
dc.contributor.author Schallig, H. D.
dc.contributor.author Büscher, P.
dc.date.accessioned 2010-08-17T12:32:10Z
dc.date.available 2010-08-17T12:32:10Z
dc.date.issued 2010
dc.identifier.issn 1935-2727
dc.identifier.doi http://dx.doi.org/10.1371/journal.pntd.0000737
dc.identifier.other ITG-P4A
dc.identifier.other ITG-PLA
dc.identifier.other PARAS
dc.identifier.other U-SEROL
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other Electronic
dc.identifier.other Abstract
dc.identifier.other FTA
dc.identifier.other UPD24
dc.identifier.uri http://hdl.handle.net/10390/6245
dc.description.abstract BACKGROUND: The polymerase chain reaction (PCR) and nucleic acid sequence-based amplification (NASBA) have been recently modified by coupling to oligochromatography (OC) for easy and fast visualisation of products. In this study we evaluate the sensitivity and specificity of the PCR-OC and NASBA-OC for diagnosis of Trypanosoma brucei gambiense and Trypanosoma brucei rhodesiense human African trypanosomiasis (HAT). METHODOLOGY AND RESULTS: Both tests were evaluated in a case-control design on 143 HAT patients and 187 endemic controls from the Democratic Republic of Congo (DRC) and Uganda. The overall sensitivity of PCR-OC was 81.8% and the specificity was 96.8%. The PCR-OC showed a sensitivity and specificity of 82.4% and 99.2% on the specimens from DRC and 81.3% and 92.3% on those from Uganda. NASBA-OC yielded an overall sensitivity of 90.2%, and a specificity of 98.9%. The sensitivity and specificity of NASBA-OC on the specimens from DRC was 97.1% and 99.2%, respectively. On the specimens from Uganda we observed a sensitivity of 84.0% and a specificity of 98.5%. ONCLUSIONS/SIGNIFICANCE: The tests showed good sensitivity and specificity for the T. b. gambiense HAT in DRC but rather a low sensitivity for T. b. rhodesiense HAT in Uganda en
dc.language English en
dc.subject Protozoal diseases en
dc.subject Trypanosomiasis, African en
dc.subject Sleeping sickness en
dc.subject Trypanosoma brucei gambiense en
dc.subject Trypanosoma brucei rhodesiense en
dc.subject Vectors en
dc.subject Tsetse flies en
dc.subject Evaluation en
dc.subject Performance en
dc.subject Rapid diagnostic tests en
dc.subject PCR en
dc.subject NASBA en
dc.subject Sensitivity en
dc.subject Specificity en
dc.subject Oligochromatography en
dc.subject Congo-Kinshasa en
dc.subject Africa, Central en
dc.subject Uganda en
dc.subject Africa, East en
dc.title Phase II evaluation of sensitivity and specificity of PCR and NASBA followed by oligochromatography for diagnosis of human African trypanosomiasis in clinical samples from D.R. Congo and Uganda en
dc.type Article-E en
dc.citation.issue 7 en
dc.citation.jtitle PLoS Neglected Tropical Diseases en
dc.citation.volume 4 en
dc.citation.pages e737 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/20625557
dc.citation.jabbreviation PLoS Negl Trop Dis en


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