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Evaluation of colorimetric methods using nicotinamide for rapid detection of pyrazinamide resistance in Mycobacterium tuberculosis

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dc.contributor.author Mirabal, N. C.
dc.contributor.author Yzquierdo, S. L.
dc.contributor.author Lemus, D.
dc.contributor.author Madruga, M.
dc.contributor.author Milián, Y.
dc.contributor.author Echemendía, M.
dc.contributor.author Takiff, H.
dc.contributor.author Martin, A.
dc.contributor.author Van der Stuyft, P.
dc.contributor.author Palomino, J. C.
dc.contributor.author Montoro, E.
dc.date.accessioned 2010-08-18T11:18:19Z
dc.date.available 2010-08-18T11:18:19Z
dc.date.issued 2010
dc.identifier.issn 0095-1137
dc.identifier.doi http://dx.doi.org/10.1128/JCM.00311-10
dc.identifier.other ITG-M8A
dc.identifier.other ITG-H9A
dc.identifier.other ITG-M10A
dc.identifier.other MULTI
dc.identifier.other MICRO
dc.identifier.other U-MYCOB
dc.identifier.other HEALTH
dc.identifier.other U-EPID
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other Abstract
dc.identifier.other UPD24
dc.identifier.uri http://hdl.handle.net/10390/6270
dc.description.abstract The direct detection of pyrazinamide resistance in Mycobacterium tuberculosis is sufficiently difficult so that many laboratories don't attempt it. Most pyrazinamide resistance is caused by mutations that inactivate the pyrazinamidase enzyme needed to convert the prodrug pyrazinamide to its active form. We evaluated two newer and simpler methods to assess pyrazinamidase activity - the nitrate reductase and malachite green microtube assays - using nicotinamide in place of pyrazinamide. A total of 102 strains were tested by these methods and the results compared with those obtained by the classic Wayne assay. Mutations in the pncA gene were identified by sequencing the pncA gene from all isolates in which pyrazinamide-resistance was detected by any of the three methods. Both the nitrate reductase and malachite green microtube assays showed sensitivities of 93.75% and specificities of 97.67%. Mutations in the pncA gene were found in 14 of 16 strains that were pyrazinamide resistant, and in 1 of 4 strains that were sensitive by the Wayne assay. Both of these simple methods, used with nicotinamide, are promising and inexpensive alternatives for the rapid detection of pyrazinamide resistance in limited-resource countries en
dc.language English en
dc.subject Bacterial diseases en
dc.subject Tuberculosis en
dc.subject Mycobacterium tuberculosis en
dc.subject Rapid diagnostic tests en
dc.subject Nicotinamide en
dc.subject Drug resistance en
dc.subject Pyrazinamide en
dc.subject Colorimetric methods en
dc.subject Evaluation en
dc.subject Strains en
dc.subject Mutations en
dc.subject Sensitivity en
dc.subject Specificity en
dc.title Evaluation of colorimetric methods using nicotinamide for rapid detection of pyrazinamide resistance in Mycobacterium tuberculosis en
dc.type Article en
dc.citation.issue 8 en
dc.citation.jtitle Journal of Clinical Microbiology en
dc.citation.volume 48 en
dc.citation.pages 2729-2733 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/20554826
dc.citation.jabbreviation J Clin Microbiol en


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