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Evaluation of four rapid tests for diagnosis and differentiation of HIV-1 and HIV-2 infections in Guinea-Conakry, West Africa

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dc.contributor.author Chaillet, P.
dc.contributor.author Tayler-Smith, K.
dc.contributor.author Zachariah, R.
dc.contributor.author Duclos, N.
dc.contributor.author Moctar, D.
dc.contributor.author Beelaert, G.
dc.contributor.author Fransen, K.
dc.date.accessioned 2010-10-05T08:14:51Z
dc.date.available 2010-10-05T08:14:51Z
dc.date.issued 2010
dc.identifier.issn 0035-9203
dc.identifier.doi http://dx.doi.org/10.1016/j.trstmh.2010.05.007
dc.identifier.other ITG-M6B
dc.identifier.other ITG-MLA
dc.identifier.other MICRO
dc.identifier.other U-HIVSTD
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other Abstract
dc.identifier.other UPD25
dc.identifier.uri http://hdl.handle.net/10390/6291
dc.description.abstract With both HIV-1 and HV-2 prevalent in Guinea-Conakry, accurate diagnosis and differentiation is crucial for treatment purposes. Thus, four rapid HIV tests were evaluated for their HIV-1 and HIV-2 diagnostic and discriminative capacity for use in Guinea-Conakry. These included SD Bioline HIV 1/2 3.0 (Standard Diagnostics Inc.), Genie II HIV1/HIV2 (Bio-Rad), First Response HIV Card Test 1-2.0 (PMC Medical) and Immunoflow HIV1-HIV2 (Core Diagnostics). Results were compared with gold standard tests (INNO-LIA HIV-I/II Score) and NEW LAV BLOT II (Bio-Rad). Four hundred and forty three sequential stored HIV-positive serum samples, of known HIV-type, were evaluated. Genie II HIV1/HIV2, Immunoflow HIV1-HIV2 and SD Bioline HIV 1/2 3.0 had 100% sensitivity (95% CI, 98.9-100%) while for First Response HIV Card Test 1-2.0 this was 99.5% (95% CI, 98.2%-99.9%). In terms of discriminatory capacity, Genie II HIV1/HIV2 identified 382/ 384(99.5%) HIV-1 samples, 49/ 52(95%) HIV-2 and 7/7(100%) HIV-positive untypable samples. Immunoflow HIV1-HIV2 identified 99% HIV-1, 67% HIV-2 and all HIV-positive untypable samples. First Response HIV Card Test 1-2.0 identified 94% HIV-1, 64% HIV-2 and 57% HIV-positive untypable samples. SD-Bioline HIV 1/2 3.0 was the worst overall performer identifying 65% HIV-1, 69% HIV-2 and all HIV-positive untypable samples. The use of SD Bioline HIV 1/2 3.0 (the current standard in Guinea-Conakry) as a discriminatory HIV test is poor and may be best replaced by Immunoflow HIV1-HIV2 en
dc.language English en
dc.subject Viral diseases en
dc.subject HIV-1 en
dc.subject HIV-2 en
dc.subject AIDS en
dc.subject Rapid diagnostic tests en
dc.subject Evaluation en
dc.subject Differentiation en
dc.subject Sensitivity en
dc.subject Specificity en
dc.subject Efficacy en
dc.subject Accuracy en
dc.subject Guinea en
dc.subject Africa, West en
dc.title Evaluation of four rapid tests for diagnosis and differentiation of HIV-1 and HIV-2 infections in Guinea-Conakry, West Africa en
dc.type Article en
dc.citation.issue 9 en
dc.citation.jtitle Transactions of the Royal Society of Tropical Medicine and Hygiene en
dc.citation.volume 104 en
dc.citation.pages 571-576 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/20708123
dc.citation.jabbreviation Trans R Soc Trop Med Hyg en


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