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Unravelling the antigenic landscape of the HIV-1 subtype A envelope of an individual with broad cross-neutralizing antibodies using phage display peptide libraries

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dc.contributor.author Dieltjens, T.
dc.contributor.author Willems, B.
dc.contributor.author Coppens, S.
dc.contributor.author Van Nieuwenhove, L.
dc.contributor.author Humbert, M.
dc.contributor.author Dietrich, U.
dc.contributor.author Heyndrickx, L.
dc.contributor.author Vanham, G.
dc.contributor.author Janssens, W.
dc.date.accessioned 2010-10-05T11:40:32Z
dc.date.available 2010-10-05T11:40:32Z
dc.date.issued 2010
dc.identifier.issn 0166-0934
dc.identifier.doi http://dx.doi.org/10.1016/j.jviromet.2010.07.004
dc.identifier.other ITG-M1B
dc.identifier.other ITG-M2B
dc.identifier.other ITG-M3B
dc.identifier.other ITG-P4B
dc.identifier.other ITG-M7A
dc.identifier.other ITG-M8A
dc.identifier.other ITG-MLA
dc.identifier.other MULTI
dc.identifier.other MICRO
dc.identifier.other U-VIROL
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other Abstract
dc.identifier.other UPD25
dc.identifier.uri http://hdl.handle.net/10390/6293
dc.description.abstract Broad cross-neutralizing antibodies from persons infected with HIV-1 target a variety of epitopes. Identification of these HIV-1 epitopes may result in an optimal panel of antigenic peptides to be included in a prophylactic vaccine. Phage display peptide libraries were used to unravel the antigenic landscape of an individual (ITM1) infected with HIV-1 subtype A with broad cross-neutralizing antibodies. A stringent selection strategy resulted in the identification of 60 unique HIV-1 peptide phage, which were subjected to sequence analysis and mapped onto the ITM1 envelope sequences. Four groups of peptide phages were found: the first group (n=11) were similar with the tip of the V3 loop (KxxHxGPxxxF); the second group (n=11) represented the gp41 principal immunodominant domain (CxGxLxCTxNxP); the third group (n=16) could be localized in the V2 loop (KxxxHxxxY); and the fourth group (n=22) mimicked a conformational epitope (Hxx(S)/(T)NxK). All but the V2-binding antibodies were conserved over the 11 years of follow-up. A neutralization inhibition assay revealed the contribution of the V3 antibodies to the neutralizing capacity of the ITM1 plasma. Overall, the ITM1 immunogenic landscape was mapped and a part of the origin of this broad cross-neutralizing activity was demonstrated en
dc.language English en
dc.subject Viral diseases en
dc.subject HIV-1 en
dc.subject AIDS en
dc.subject Cross-neutralization en
dc.subject Antibodies en
dc.subject Epitopes en
dc.subject Identification en
dc.subject Peptides en
dc.title Unravelling the antigenic landscape of the HIV-1 subtype A envelope of an individual with broad cross-neutralizing antibodies using phage display peptide libraries en
dc.type Article en
dc.citation.issue 1 en
dc.citation.jtitle Journal of Virological Methods en
dc.citation.volume 169 en
dc.citation.pages 95-102 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/20637241
dc.citation.jabbreviation J Virol Meth en


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