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Development of an enzyme-linked immunosorbent assay to identify host-feeding preferences of Phlebotomus species (Diptera: Psychodidae) in endemic foci of visceral leishmaniasis in Nepal

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dc.contributor.author Burniston, I.
dc.contributor.author Roy, L.
dc.contributor.author Picado, A.
dc.contributor.author Das, M.
dc.contributor.author Rijal, S.
dc.contributor.author Rogers, M.
dc.contributor.author Coosemans, M.
dc.contributor.author Boelaert, M.
dc.contributor.author Davies, C.
dc.contributor.author Cameron, M.
dc.date.accessioned 2010-10-27T13:48:26Z
dc.date.available 2010-10-27T13:48:26Z
dc.date.issued 2010
dc.identifier.issn 0022-2585
dc.identifier.doi http://dx.doi.org/10.1603/ME09184
dc.identifier.other ITG-P7A
dc.identifier.other ITG-H8A
dc.identifier.other MULTI
dc.identifier.other PARAS
dc.identifier.other U-ENTOM
dc.identifier.other HEALTH
dc.identifier.other U-EPID
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other UPD26
dc.identifier.other FTC
dc.identifier.uri http://hdl.handle.net/10390/6335
dc.description.abstract Anthroponotic visceral leishmaniasis, transmitted by Phlebotomus argentipes Annandale & Brunetti (Diptera: Psychodidae) sand flies, is regarded as a major problem of public health importance in the Indian subcontinent. Understanding the feeding behavior of the vector can be used to investigate changes in human-vector contact during intervention programs. An enzyme-linked immunosorbent assay (ELISA) was modified to make it suitable to identify the origin of P. argentipes and Phlebotomus papatasi Scopoli (Diptera: Psychodidae) blood meals. The sensitivity and specificity of the precipitin ring test and ELISA were compared, as well as the stability of the tests across different stages of blood meal digestion. The ELISA was more sensitive and specific than the precipitin test for identifying the sources of blood meals. When using the ELISA method with a plate reader, it was possible to obtain 100% sensitivity and specificity. When comparing the techniques across digestion stages, it was found that there was a drop in sensitivity, 48 and 72 h postblood meal for precipitin and visually read ELISA, respectively. However, the sensitivity of the ELISA using a plate reader was not altered by the digestion time. The feeding habits of P. argentipes and P. papatasi from the Terai region of Nepal, determined by the ELISA developed, showed P. papatasi to be highly anthropophilic, and P. argentipes appeared to feed both on humans and animals, in particular bovines. en
dc.language English en
dc.subject Protozoal diseases en
dc.subject Leishmaniasis en
dc.subject Visceral en
dc.subject Kala azar en
dc.subject Leishmania donovani en
dc.subject Vectors en
dc.subject Sandflies en
dc.subject Phlebotomus argentipes en
dc.subject Phlebotomus papatasi en
dc.subject Vector ecology en
dc.subject Identification en
dc.subject Enzyme-linked immunosorbent assay (ELISA) en
dc.subject ELISA en
dc.subject Sensitivity en
dc.subject Specificity en
dc.subject Feeding habits en
dc.subject Feeding behavior en
dc.subject Nepal en
dc.subject Asia, South en
dc.title Development of an enzyme-linked immunosorbent assay to identify host-feeding preferences of Phlebotomus species (Diptera: Psychodidae) in endemic foci of visceral leishmaniasis in Nepal en
dc.type Article en
dc.citation.issue 5 en
dc.citation.jtitle Journal of Medical Entomology en
dc.citation.volume 47 en
dc.citation.pages 902-906 en
dc.citation.jabbreviation J Med Entomol en


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