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Methylene blue is a good background stain for tuberculosis light-emitting diode fluorescence microscopy

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dc.contributor.author Van Deun, A.
dc.contributor.author Aung, K. J.
dc.contributor.author Hamid Salim, A.
dc.contributor.author Gumusboga, M.
dc.contributor.author Nandi, P.
dc.contributor.author Hossain, M. A.
dc.date.accessioned 2011-01-06T14:07:42Z
dc.date.available 2011-01-06T14:07:42Z
dc.date.issued 2010
dc.identifier.issn 1027-3719
dc.identifier.other ITG-M1A
dc.identifier.other ITG-M4B
dc.identifier.other MICRO
dc.identifier.other U-MYCOB
dc.identifier.other JIF
dc.identifier.other Abstract
dc.identifier.other UPD29
dc.identifier.uri http://hdl.handle.net/10390/6428
dc.description.abstract SETTING: Damien Foundation Bangladesh tuberculosis (TB) control projects. OBJECTIVES: To compare blue ink, potassium permanganate and methylene blue background staining for transmitted light-emitting diode (LED) TB fluorescence microscopy (FM). DESIGN: Auramine smears made in triplicate from Ziehl-Neelsen (ZN) acid-fast bacilli (AFB) positive or negative sputum and stained with one of the background variations were read blind by LED FM. Reference laboratory rechecking of discordant series was used before and after auramine restaining as the gold standard. RESULTS: Of 1977 series evaluated, 991 (50.1%) were made from ZN-positive specimens. There were 919, 942 and 958 FM true-positives with blue ink, permanganate and methylene blue counterstaining, against respectively 12, 12 and 16 false-positives. Methylene blue counterstaining was more sensitive (95.6%, 95%CI 94.2-96.8) than blue ink or permanganate (92.7%, 95%CI 90.9-94.3 and 93.6%, 95%CI 91.9-95.0; respectively P < 0.01 and <0.05). No AFB could be found in 85% and 18% of 180 discordant series without and with restaining. CONCLUSIONS: Methylene blue is at least equivalent to potassium permanganate counterstaining for FM using blue LED transmitted excitation and is cheaper than blue ink. Restaining of all smears prior to first re-reading may be unavoidable for blinded rechecking of auramine-stained smears for external quality assessment. en
dc.language English en
dc.subject Bacterial diseases en
dc.subject Tuberculosis en
dc.subject Mycobacterium tuberculosis en
dc.subject Diagnosis en
dc.subject Rapid diagnostic tests en
dc.subject Comparison en
dc.subject Fluorescence microscopy en
dc.subject Light microscopy en
dc.subject Potassium en
dc.subject Methylene blue en
dc.subject Staining en
dc.subject Ziehl-Neelsen smear en
dc.subject Acid-fast bacilli en
dc.subject Sputum microscopy en
dc.subject Auramine en
dc.subject False-positive en
dc.subject Sensitivity en
dc.subject Laboratory techniques and procedures en
dc.title Methylene blue is a good background stain for tuberculosis light-emitting diode fluorescence microscopy en
dc.type Article en
dc.citation.issue 12 en
dc.citation.jtitle International Journal of Tuberculosis and Lung Disease en
dc.citation.volume 14 en
dc.citation.pages 1571-1575 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/21144242
dc.citation.jabbreviation Int J Tuberc Lung Dis en


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