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Leishmania AFLP: paving the way towards improved molecular assays and markers of diversity

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dc.contributor.author Odiwuor, S.
dc.contributor.author Vuylsteke, M.
dc.contributor.author De Doncker, S.
dc.contributor.author Maes, I.
dc.contributor.author Mbuchi, M.
dc.contributor.author Dujardin, J. C.
dc.contributor.author Van der Auwera, G.
dc.date.accessioned 2011-06-01T12:18:54Z
dc.date.available 2011-06-01T12:18:54Z
dc.date.issued 2011
dc.identifier.issn 1567-1348
dc.identifier.doi http://dx.doi.org/10.1016/j.meegid.2011.03.008
dc.identifier.other ITG-P1B
dc.identifier.other ITG-P3B
dc.identifier.other ITG-P4B
dc.identifier.other ITG-P6A
dc.identifier.other ITG-PLB
dc.identifier.other PARAS
dc.identifier.other U-PROTO
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other Abstract
dc.identifier.uri http://hdl.handle.net/10390/6586
dc.description.abstract Diversity, phylogenetic, and population genetic studies of the genus Leishmania, causative agent of leishmaniasis, nowadays generally involves multilocus microsatellite and multilocus sequence typing. Even though these are well established and useful applications, amplified fragment length polymorphisms (AFLP) can provide complementary information. In addition, as the technique essentially probes the entire genome at random, without prior sequence knowledge, it is ideally suited as a screening tool for molecular markers linked with biological and clinical traits. We developed an AFLP protocol adapted to the Leishmania genome, tested its repeatability, and validated it on a panel of samples from the Leishmania donovani complex previously analyzed by multiple molecular tests. The technique proved highly reproducible, and showed that genetic relationships between L. donovani strains generally reflect geographic distance. Four main groups were identified: L. infantum, African L. donovani, Indian L. donovani, and a mixed group consisting of L. donovani from India and Africa. Results were highly congruent with previous analyses on essentially the same sample set, indicating that the developed assay produces trustworthy data. This opens possibilities for application in studies of speciation and population dynamics. Moreover, it allows random screening of the entire Leishmania genome for linkage with biological and clinical parasite properties, such as fitness, drug resistance, and disease profile. en
dc.language English en
dc.subject Protozoal diseases en
dc.subject Leishmaniasis en
dc.subject Leishmania donovani en
dc.subject Leishmania infantum en
dc.subject Leishmania archibaldi en
dc.subject Leishmania chagasi en
dc.subject Molecular epidemiology en
dc.subject Genetic diversity en
dc.subject Strains en
dc.subject Phylogeny en
dc.subject Primers en
dc.subject AFLP en
dc.subject Molecular en
dc.subject Assays en
dc.subject Markers en
dc.subject Fingerprinting en
dc.subject Protocols en
dc.subject Reproducibility en
dc.subject Repeatability en
dc.subject Laboratory techniques and procedures en
dc.title Leishmania AFLP: paving the way towards improved molecular assays and markers of diversity en
dc.type Article en
dc.citation.issue 5 en
dc.citation.jtitle Infection, Genetics and Evolution en
dc.citation.volume 11 en
dc.citation.pages 960-967 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/21439405
dc.citation.jabbreviation Infect Genet Evol en


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