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Post-mortem examination and laboratory-based analysis for the diagnosis of bovine tuberculosis among dairy cattle in Ecuador

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dc.contributor.author Proaño-Perez, F.
dc.contributor.author Benitez-Ortiz, W.
dc.contributor.author Desmecht, D.
dc.contributor.author Coral, M.
dc.contributor.author Ortiz, J.
dc.contributor.author Ron, L.
dc.contributor.author Portaels, F.
dc.contributor.author Rigouts, L.
dc.contributor.author Linden, A.
dc.date.accessioned 2011-06-30T09:23:32Z
dc.date.available 2011-06-30T09:23:32Z
dc.date.issued 2011
dc.identifier.issn 0167-5877
dc.identifier.doi http://dx.doi.org/10.1016/j.prevetmed.2011.04.018
dc.identifier.other ITG-M7A
dc.identifier.other ITG-M8A
dc.identifier.other MICRO
dc.identifier.other U-MYCOB
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other Abstract
dc.identifier.other UPD35
dc.identifier.uri http://hdl.handle.net/10390/6605
dc.description.abstract Veterinary inspection in slaughterhouses allows for the detection of macroscopic lesions reminiscent of bovine tuberculosis, but the presence of Mycobacterium bovis must be confirmed by laboratory methods. This study aimed at comparing the performances of the standard diagnostic tools used to identify M. bovis in tissue specimens sampled from suspicious animals. During a two years period, 1390 cattle were inspected at the Machachi abattoir in the Mejia canton - Ecuador. A total of 33 animals with granulomatous lesions were detected, representing 2.33% (16/687) and 2.42% (17/703) animals examined in 2007 and 2008, respectively. Ninety-four tissue specimens were sampled and screened for the presence of mycobacteria. Acid-fast bacilli were identified in one third of the suspicious cattle (11/33) and suggestive microscopic lesions in 27.3% (9/33) of the samples examined by direct microscopy and histopathology, respectively. Culturing on Stonebrink medium and 16S-rRNA-based polymerase chain reaction (PCR) yielded 36.4% (12/33) and 27.3% (9/33) of positives, respectively. Compared to culture, other diagnostic procedures displayed a lower sensitivity, with 56.5% for PCR, and 43.5% for direct microscopy and histopathology; however, the specificity was higher (94.4% for PCR and microscopy, and 97.2% for histopathology). We conclude that reliable post-mortem laboratory testing either requires the combination of a set of available diagnostic tools or necessitates the development of improved new-generation tools with better sensitivity and specificity characteristics. en
dc.language English en
dc.subject Bacterial diseases en
dc.subject Bovine en
dc.subject Tuberculosis en
dc.subject Mycobacterium bovis en
dc.subject Cattle en
dc.subject Diagnostics en
dc.subject Tools en
dc.subject Comparison en
dc.subject Performance en
dc.subject Granuloma en
dc.subject Screening en
dc.subject Acid-fast bacilli en
dc.subject Culture techniques en
dc.subject Polymerase chain reaction en
dc.subject PCR en
dc.subject Sensitivity en
dc.subject Specificity en
dc.subject Combinations en
dc.subject Laboratory techniques and procedures en
dc.subject Ecuador en
dc.subject America, Latin en
dc.title Post-mortem examination and laboratory-based analysis for the diagnosis of bovine tuberculosis among dairy cattle in Ecuador en
dc.type Article en
dc.citation.issue 1-2 en
dc.citation.jtitle Preventive Veterinary Medicine en
dc.citation.volume 101 en
dc.citation.pages 65-72 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/21645934
dc.citation.jabbreviation Prev Vet Med en


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