Institute of Tropical Medicine Antwerp
Foundation of Public Utility

Field evaluation of urine antigen detection for diagnosis of Taenia solium cysticercosis

DSpace/Manakin Repository

Show simple item record Mwape, K. E. Praet, N. Benitez-Ortiz, W. Muma, J. B. Zulu, G. Celi-Erazo, M. Phiri, I. K. Rodriguez-Hidalgo, R. Dorny, P. Gabriel, S. 2011-10-05T12:33:50Z 2011-10-05T12:33:50Z 2011
dc.identifier.issn 0035-9203
dc.identifier.other ITG-A2A
dc.identifier.other ITG-ALA
dc.identifier.other ANIMAL
dc.identifier.other U-VHELM
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other Abstract
dc.identifier.other UPD38
dc.description.abstract (Neuro)cysticercosis is an important zoonotic disease caused by infection with Taenia solium metacestode larvae. Existing immunodiagnostic techniques detect antibodies and circulating antigens (Ag) in serum and cerebrospinal fluid (CSF). Blood/CSF collection is an invasive procedure associated with blood-borne infections and is often not well accepted by communities. Detection of circulating Ag in urine has been suggested as an alternative, however this has been evaluated in clinical settings only. The aim of the present study was to evaluate the performance of a urine Ag-ELISA under field conditions. Paired serum and urine samples were obtained from participants in endemic areas of Ecuador (n=748) and Zambia (n=690) and were subjected to a monoclonal antibody-based Ag-ELISA. Calculation of positive and negative agreement indices (AI) showed better agreement in the negative direction both for Ecuadorian and Zambian samples (AI of 93.1 and 86.8, respectively). Using a Bayesian approach to determine the test characteristics, similar sensitivities were obtained for serum and urine Ag detection, whereas a decreased specificity was determined for the urine Ag-ELISA with a lower specificity (78.6%) for Zambian samples than for Ecuadorian samples (88.4%). This study indicates a higher specificity for the serum test under field conditions and promotes further research to improve the urine test. en
dc.language English en
dc.subject Helminthic diseases en
dc.subject Taenia solium en
dc.subject Taeniasis en
dc.subject Neurocysticercosis en
dc.subject Diagnosis en
dc.subject Rapid diagnostic tests en
dc.subject Antigen detection en
dc.subject Urine en
dc.subject Cerebrospinal fluid en
dc.subject Evaluation en
dc.subject Performance en
dc.subject Ag-ELISA en
dc.subject Monoclonal antibodies en
dc.subject Specificity en
dc.subject Sensitivity en
dc.subject Zambia en
dc.subject Africa, Southern en
dc.subject Ecuador en
dc.subject America, Latin en
dc.title Field evaluation of urine antigen detection for diagnosis of Taenia solium cysticercosis en
dc.type Article en
dc.citation.issue 10 en
dc.citation.jtitle Transactions of the Royal Society of Tropical Medicine and Hygiene en
dc.citation.volume 105 en
dc.citation.pages 574-578 en
dc.citation.jabbreviation Trans R Soc Trop Med Hyg en

Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record