Institute of Tropical Medicine Antwerp
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Newly developed primers for the detection of Mycobacterium avium subspecies paratuberculosis

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dc.contributor.author Vansnick, E. en_US
dc.contributor.author de Rijk, P. en_US
dc.contributor.author Vercammen, F. en_US
dc.contributor.author Geysen, D. en_US
dc.contributor.author Rigouts, L. en_US
dc.contributor.author Portaels, F. en_US
dc.date.accessioned 2007-12-06T14:37:03Z
dc.date.available 2007-12-06T14:37:03Z
dc.date.issued 2004 en_US
dc.identifier.issn 0378-1135 en_US
dc.identifier.doi http://dx.doi.org/10.1016/j.vetmic.2004.02.006
dc.identifier.other ITG-A1A en_US
dc.identifier.other ITG-M2B en_US
dc.identifier.other ITG-A4A en_US
dc.identifier.other ITG-M5A en_US
dc.identifier.other ITG-MLA en_US
dc.identifier.other ANIMAL en_US
dc.identifier.other U-ANIMAL en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-MYCOB en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other MULTI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.uri http://hdl.handle.net/10390/670
dc.description.abstract Recent publications reported the existence of IS900 like sequences in mycobacteria different from Mycobacterium avium subspecies paratuberculosis (Map). The primers used for IS900 detection of Map have amplified these sequences causing false positive results. In this study, we have developed two new PCR assays for the detection of Map. The first assay is based on the IS900 sequence using primers different from the ones previously reported, the second assay on the f57 sequence. The specificity of the tests was checked by analysis of 190 mycobacterial isolates (74 Map and 116 non-Map isolates). All Map strains were positive and all non-Map strains were negative. Serial dilutions of Map bacteria were used to assess the sensitivity of the assays. We achieved a sensitivity of 1CFU per PCR for both assays. In addition, a PCR-simulating computer programme was used to evaluate the specificity of the new IS900 primers. The combination of the two PCR assays has proven to be useful for the identification of Map but validation on a large range of clinical samples still needs to be done. en_US
dc.language English en_US
dc.subject Animal diseases en_US
dc.subject Bacterial diseases en_US
dc.subject Johne's disease en_US
dc.subject Mycobacterium avium paratuberculosis en_US
dc.subject Molecular diagnostic techniques en_US
dc.subject PCR en_US
dc.subject Identification en_US
dc.subject Sensitivity en_US
dc.subject Specificity en_US
dc.subject IS900 en_US
dc.subject f57 en_US
dc.subject Primers en_US
dc.title Newly developed primers for the detection of Mycobacterium avium subspecies paratuberculosis en_US
dc.type Article en_US
dc.citation.issue 3-4 en_US
dc.citation.jtitle Veterinary Microbiology en_US
dc.citation.volume 100 en_US
dc.citation.pages 197-204 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/15145498
dc.citation.jabbreviation Vet Microbiol en_US


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