Institute of Tropical Medicine Antwerp
Foundation of Public Utility

A DNA sequence capture extraction method for detection of Mycobacterium avium subspecies paratuberculosis in feces and tissue samples

DSpace/Manakin Repository

Show simple item record

dc.contributor.author Vansnick, E. en_US
dc.contributor.author de Rijk, P. en_US
dc.contributor.author Vercammen, F. en_US
dc.contributor.author Rigouts, L. en_US
dc.contributor.author Portaels, F. en_US
dc.contributor.author Geysen, D. en_US
dc.date.accessioned 2007-12-06T14:37:04Z
dc.date.available 2007-12-06T14:37:04Z
dc.date.issued 2007 en_US
dc.identifier.issn 0378-1135 en_US
dc.identifier.doi http://dx.doi.org/10.1016/j.vetmic.2007.01.011
dc.identifier.other ITG-A1B en_US
dc.identifier.other ITG-M2B en_US
dc.identifier.other ITG-M4A en_US
dc.identifier.other ITG-M5A en_US
dc.identifier.other ITG-ALA en_US
dc.identifier.other ANIMAL en_US
dc.identifier.other U-ANIMAL en_US
dc.identifier.other MICRO en_US
dc.identifier.other U-MYCOB en_US
dc.identifier.other JIF en_US
dc.identifier.other DOI en_US
dc.identifier.other ABSTRACT en_US
dc.identifier.other MULTI en_US
dc.identifier.uri http://hdl.handle.net/10390/671
dc.description.abstract Culturing of Mycobacterium avium subspecies paratuberculosis (Map) remains difficult and is time consuming. An alternative for the rapid detection of Map in samples is PCR. We have developed a sensitive DNA-extraction method based on sequence capture for the rapid detection of M. avium subspecies paratuberculosis by PCR in fecal and tissue samples. The method detected 10(2)Map/g feces using spiked samples, and reached a diagnostic sensitivity of 33,7% compared to 22% for culture. Analysis of tissue samples gave 65 polymerase chain reaction (PCR)-positive (42.2%) and 49 culture-positive samples (31.8%). Therefore, the detection limit of the DNA-extraction is the same as previously reported for culture, the PCR assay could detect more positive samples than the culture method. en_US
dc.language English en_US
dc.subject Microbiology en_US
dc.subject Mycobacterium avium paratuberculosis en_US
dc.subject Laboratory diagnosis en_US
dc.subject Feces en_US
dc.subject DNA extraction en_US
dc.title A DNA sequence capture extraction method for detection of Mycobacterium avium subspecies paratuberculosis in feces and tissue samples en_US
dc.type Article en_US
dc.citation.issue 1-2 en_US
dc.citation.jtitle Veterinary Microbiology en_US
dc.citation.volume 122 en_US
dc.citation.pages 166-171 en_US
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/17329043
dc.citation.jabbreviation Vet Microbiol en_US


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record