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Theileria parva isolate of low virulence infects a subpopulation of lymphocytes

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Show simple item record Tindih, H. S. Geysen, D. Goddeeris, B. M. Awino, E. Dobbelaere, D. A. Naessens, J. 2012-02-29T14:36:49Z 2012-02-29T14:36:49Z 2012
dc.identifier.issn 0019-9567
dc.identifier.other ITG-B1B
dc.identifier.other ITG-B2A
dc.identifier.other DBM
dc.identifier.other U-VEPID
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other UPD43
dc.description.abstract Theileria parva is a tick-transmitted protozoan parasite that infects and transforms bovine lymphocytes. We have previously shown that Theileria parvaChitongo (TpC) is an isolate of lower virulence than T.parvaMuguga (TpM). Lower virulence appeared correlated with a delayed onset of thelogarithmic growth phase of TpC-tranformedperipheral blood mononuclear cells after in vitro infection. In the current study, infection experiments of WC1(+)-gammadelta-T cellsrevealed thatonly TpMcould infect these cells andthat no transformed cells could be obtained withTpC sporozoites. Subsequent analysis of susceptibility of different cell lines and purified populations of lymphocytes for infection and transformation by both isolates showed that TpMsporozoites could attach and infectCD4(+), CD8(+) and WC1(+) T lymphocytes, but that TpCsporozoites were only observed to bind to the CD8(+) T cell population. Flow cytometry analysis of established,transformed clones confirmed this bias in target cells. TpM-transformed clones consisted of different cell surface phenotypes, suggesting that they were derived from either host CD4(+), CD8(+)or WC1(+)T cells. In contrast, all in vitro and in vivoTpC-transformed clones expressed CD8 but not CD4 or WC1, suggesting that the TpC-transformed target cells were exclusivelyinfected CD8(+) lymphocytes.So a role of cell tropism in virulence is likely. Since the adhesion molecule p67 is 100 % identical between the two strains, a second, high affinity adhesin that determines target cell specificity appears to exist. en
dc.language English en
dc.subject Animal diseases en
dc.subject Protozoal diseases en
dc.subject Theileriasis en
dc.subject East Coast fever en
dc.subject Theileria parva en
dc.title Theileria parva isolate of low virulence infects a subpopulation of lymphocytes en
dc.type Article en
dc.citation.issue 3 en
dc.citation.jtitle Infection and Immunity en
dc.citation.volume 80 en
dc.citation.pages 1267-1273 en
dc.citation.jabbreviation Infect Immun en

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