Institute of Tropical Medicine Antwerp
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Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture

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dc.contributor.author Borlon, C.
dc.contributor.author Russell, B.
dc.contributor.author Sriprawat, K.
dc.contributor.author Suwanarusk, R.
dc.contributor.author Erhart, A.
dc.contributor.author Renia, L.
dc.contributor.author Nosten, F.
dc.contributor.author D'Alessandro, U.
dc.date.accessioned 2012-02-29T14:46:24Z
dc.date.available 2012-02-29T14:46:24Z
dc.date.issued 2012
dc.identifier.issn 0020-7519
dc.identifier.doi http://dx.doi.org/10.1016/j.ijpara.2011.10.011
dc.identifier.other ITG-B1B
dc.identifier.other ITG-B5A
dc.identifier.other ITG-BLA
dc.identifier.other DBM
dc.identifier.other U-MALAR
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other Abstract
dc.identifier.other UPD43
dc.identifier.uri http://hdl.handle.net/10390/6834
dc.description.abstract The establishment of a Plasmodium vivaxin vitro culture system is critical for the development of new vaccine, drugs and diagnostic tests. Although short-term cultures have been successfully set up, their reproducibility in laboratories without direct access to P. vivax-infected patients has been limited by the need for fresh parasite isolates. We explored the possibility of using parasite isolates and reticulocytes, both cryopreserved, to perform invasion and initiate short-term culture. Invasion results obtained with both cryopreserved isolates and reticulocytes were similar to those obtained with fresh samples. This method should be easily replicated in laboratories outside endemic areas and will substantially contribute to the development of a continuous P. vivax culture. In addition, this model could be used for testing vaccine candidates as well as for studying invasion-specific molecular mechanisms. en
dc.language English en
dc.subject Protozoal diseases en
dc.subject Malaria en
dc.subject Plasmodium vivax en
dc.subject Vectors en
dc.subject Mosquitoes en
dc.subject Anopheles en
dc.subject Diagnostics en
dc.subject Research en
dc.subject Vaccine development en
dc.subject Drug development en
dc.subject Culture techniques en
dc.subject Short term en
dc.subject In vitro en
dc.subject Cryopreservation en
dc.subject Reticulocytes en
dc.subject Blood en
dc.subject Reproducibility en
dc.subject Laboratory techniques and procedures en
dc.title Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture en
dc.type Article en
dc.citation.issue 2 en
dc.citation.jtitle International Journal for Parasitology en
dc.citation.volume 42 en
dc.citation.pages 155-160 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/22240310
dc.citation.jabbreviation Int J Parasitol en


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