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hsp65 PCR-restriction analysis (PRA) with capillary electrophoresis for species identification and differentiation of Mycobacterium kansasii and Mycobacterium chelonae-Mycobacterium abscessus group

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dc.contributor.author Sajduda, A.
dc.contributor.author Martin, A.
dc.contributor.author Portaels, F.
dc.contributor.author Palomino, J. C.
dc.date.accessioned 2012-02-29T14:54:26Z
dc.date.available 2012-02-29T14:54:26Z
dc.date.issued 2012
dc.identifier.issn 1201-9712
dc.identifier.doi http://dx.doi.org/10.1016/j.ijid.2011.11.011
dc.identifier.other ITG-B1B
dc.identifier.other ITG-B2A
dc.identifier.other ITG-B3A
dc.identifier.other ITG-BLA
dc.identifier.other DBM
dc.identifier.other U-MYCOB
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other UPD43
dc.identifier.uri http://hdl.handle.net/10390/6835
dc.description.abstract OBJECTIVES: The aim of the present study was to identify and differentiate Mycobacterium kansasii and Mycobacterium chelonae-Mycobacterium abscessus group strains isolated from clinical and environmental sources in different countries. METHODS: PCR-restriction analysis of the hsp65 gene (PRA) with automated capillary electrophoresis was applied to the isolates previously identified by conventional biochemical testing and the molecular INNO-LiPA MYCOBACTERIA assay. RESULTS: PRA performed very well in comparison with the two other methods (96.4% concordance). Among 27M. kansasii isolates, this method detected five genetic types, of which type 1 represented the most common clinical isolate, as it is worldwide. PRA differentiated 29M. chelonae-M. abscessus group isolates into Mycobacterium immunogenum type 2 (n=13), M. chelonae (n=12), and M. abscessus types 1 (n=1) and 2 (n=1). M. immunogenum was the most frequent (69%) isolate from humans, but only one of 11 cases was clinically significant. M. chelonae was the most commonly (83%) recovered from water. PRA also identified two isolates with hsp65 alleles representing previously unreported patterns. CONCLUSIONS: PRA based on automated capillary electrophoresis is a rapid, simple, and reliable method for the identification and differentiation of both clinically relevant and environmental isolates of M. kansasii and M. chelonae-M. abscessus group. en
dc.language English en
dc.subject Bacterial diseases en
dc.subject Tuberculosis en
dc.subject Leprosy en
dc.subject Mycobacterium kansasii en
dc.subject Mycobacterium chelonae en
dc.subject Mycobacterium abscessus en
dc.subject Molecular diagnostic techniques en
dc.subject Species en
dc.subject Identification en
dc.subject Differentiation en
dc.subject Polymerase chain reaction en
dc.subject PCR-restriction analysis en
dc.subject Electrophoresis en
dc.subject Laboratory techniques and procedures en
dc.title hsp65 PCR-restriction analysis (PRA) with capillary electrophoresis for species identification and differentiation of Mycobacterium kansasii and Mycobacterium chelonae-Mycobacterium abscessus group en
dc.type Article en
dc.citation.issue 3 en
dc.citation.jtitle International Journal of Infectious Diseases en
dc.citation.volume 16 en
dc.citation.pages e193-e197 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/22240012
dc.citation.jabbreviation Int J Infect Dis en


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