Keywords:
Protozoal diseases
Cutaneous
Leishmaniasis
Leishmania braziliensis
Leishmania guyanensis
Vectors
Sandflies
Lutzomyia
Identification
Isolation
Algorithms
Polymerase chain reaction
PCR
Amplification
Performance
Peru
America, Latin
Abstract:
We present an algorithm based on three PCR assays for Leishmania (Viannia) species identification and assessed its performance using 70 specimens from Peruvian patients. The succession of the assayed targets can be ordered according to species prevalence. Sequential progression through the algorithm reduced the number of samples here studied by approximately 30% after each step.