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Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis in Peru: assessment of a molecular step-wise approach

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dc.contributor.author Veland, N.
dc.contributor.author Boggild, A. K.
dc.contributor.author Valencia, C.
dc.contributor.author Valencia, B. M.
dc.contributor.author Llanos-Cuentas, A.
dc.contributor.author Van der Auwera, G.
dc.contributor.author Dujardin, J. C.
dc.contributor.author Arevalo, J.
dc.date.accessioned 2012-02-29T15:15:05Z
dc.date.available 2012-02-29T15:15:05Z
dc.date.issued 2012
dc.identifier.issn 0095-1137
dc.identifier.doi http://dx.doi.org/10.1128/JCM.05061-11
dc.identifier.other ITG-B6A
dc.identifier.other ITG-B7A
dc.identifier.other DBM
dc.identifier.other U-MOLPAR
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other UPD43
dc.identifier.uri http://hdl.handle.net/10390/6837
dc.description.abstract We present an algorithm based on three PCR assays for Leishmania (Viannia) species identification and assessed its performance using 70 specimens from Peruvian patients. The succession of the assayed targets can be ordered according to species prevalence. Sequential progression through the algorithm reduced the number of samples here studied by approximately 30% after each step. en
dc.language English en
dc.subject Protozoal diseases en
dc.subject Cutaneous en
dc.subject Leishmaniasis en
dc.subject Leishmania braziliensis en
dc.subject Leishmania guyanensis en
dc.subject Vectors en
dc.subject Sandflies en
dc.subject Lutzomyia en
dc.subject Identification en
dc.subject Isolation en
dc.subject Algorithms en
dc.subject Polymerase chain reaction en
dc.subject PCR en
dc.subject Amplification en
dc.subject Performance en
dc.subject Peru en
dc.subject America, Latin en
dc.title Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis in Peru: assessment of a molecular step-wise approach en
dc.type Article en
dc.citation.issue 2 en
dc.citation.jtitle Journal of Clinical Microbiology en
dc.citation.volume 50 en
dc.citation.pages 495-498 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/22116151
dc.citation.jabbreviation J Clin Microbiol en


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