Institute of Tropical Medicine Antwerp
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Polymeric multilayer capsule-mediated vaccination induces protective immunity against cancer and viral infection

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dc.contributor.author De Geest, B. G.
dc.contributor.author Willart, M. A.
dc.contributor.author Hammad, H.
dc.contributor.author Lambrecht, B. N.
dc.contributor.author Pollard, C.
dc.contributor.author Bogaert, P.
dc.contributor.author De Filette, M.
dc.contributor.author Saelens, X.
dc.contributor.author Vervaet, C.
dc.contributor.author Remon, J. P.
dc.contributor.author Grooten, J.
dc.contributor.author De Koker, S.
dc.date.accessioned 2012-04-25T13:37:43Z
dc.date.available 2012-04-25T13:37:43Z
dc.date.issued 2012
dc.identifier.issn 1936-0851
dc.identifier.doi http://dx.doi.org/10.1021/nn205099c
dc.identifier.other ITG-B5B
dc.identifier.other DBM
dc.identifier.other U-VIROL
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other Abstract
dc.identifier.other UPD45
dc.identifier.uri http://hdl.handle.net/10390/6951
dc.description.abstract Recombinant antigens hold high potential to develop vaccines against lethal intracellular pathogens and cancer. However, they are poorly immunogenic and fail to induce potent cellular immunity. In this paper, we demonstrate that polymeric multilayer capsules (PMLC) strongly increase antigen delivery toward professional antigen-presenting cells in vivo, including dendritic cells (DCs), macrophages, and B cells, thereby enforcing antigen presentation and stimulating T cell proliferation. A thorough analysis of the T cell response demonstrated their capacity to induce IFN-gamma secreting CD4 and CD8 T cells, in addition to follicular T-helper cells, a recently identified CD4 T cell subset supporting antibody responses. On the B cell level, PMLC-mediated antigen delivery promoted the formation of germinal centers, resulting in increased numbers of antibody-secreting plasma cells and elevated antibody titers. The functional relevance of the induced immune responses was validated in murine models of influenza and melanoma. On a mechanistic level, we have demonstrated the capacity of PMLC to activate the NALP3 inflammasome and trigger the release of the potent pro-inflammatory cytokine IL-1beta. Finally, using DC-depleted mice, we have identified DCs as the key mediators of the immunogenic properties of PMLC. en
dc.language English en
dc.subject Viral diseases en
dc.subject Cancer en
dc.subject Vaccine development en
dc.subject Immunochemistry en
dc.subject Polyelectrolyte multilayer capsules en
dc.subject Immunity en
dc.subject Dendritic cells en
dc.subject Macrophages en
dc.subject B-cells en
dc.subject T-cells en
dc.subject CD4 en
dc.subject CD8 en
dc.subject Antibodies en
dc.subject Cytokine production en
dc.title Polymeric multilayer capsule-mediated vaccination induces protective immunity against cancer and viral infection en
dc.type Article en
dc.citation.issue 3 en
dc.citation.jtitle ACS Nano en
dc.citation.volume 6 en
dc.citation.pages 2136-2149 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/22303914
dc.citation.jabbreviation ACS Nano en


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