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Interleukin-12p70 expression by dendritic cells of HIV-1-infected patients fails to stimulate gag-specific immune responses

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dc.contributor.author Van Gulck, E.
dc.contributor.author Cools, N.
dc.contributor.author Atkinson, D.
dc.contributor.author Bracke, L.
dc.contributor.author Vereecken, K.
dc.contributor.author Vekemans, M.
dc.contributor.author Van Tendeloo, V. F. I.
dc.contributor.author Berneman, Z. N.
dc.contributor.author Vanham, G.
dc.date.accessioned 2012-10-04T15:40:51Z
dc.date.available 2012-10-04T15:40:51Z
dc.date.issued 2012
dc.identifier.issn 1740-2522
dc.identifier.doi http://dx.doi.org/10.1155/2012/184979
dc.identifier.other ITG-B1B
dc.identifier.other ITG-B3B
dc.identifier.other ITG-B4B
dc.identifier.other ITG-B5B
dc.identifier.other ITG-C6A
dc.identifier.other ITG-BLA
dc.identifier.other MULTI
dc.identifier.other DBM
dc.identifier.other U-VIROL
dc.identifier.other U-TRAVC
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other FTA
dc.identifier.other Abstract
dc.identifier.other UPD52
dc.identifier.uri http://hdl.handle.net/10390/7166
dc.description.abstract A variety of immune-based therapies has been developed in order to boost or induce protective CD8(+) T cell responses in order to control HIV replication. Since dendritic cells (DCs) are professional antigen-presenting cells (APCs) with the unique capability to stimulate na‹ve T cells into effector T cells, their use for the induction of HIV-specific immune responses has been studied intensively. In the present study we investigated whether modulation of the activation state of DCs electroporated with consensus codon-optimized HxB2 gag mRNA enhances their capacity to induce HIV gag-specific T cell responses. To this end, mature DCs were (i) co-electroporated with mRNA encoding interleukin (IL)-12p70 mRNA, or (ii) activated with a cytokine cocktail consisting of R848 and interferon (IFN)-?. Our results confirm the ability of HxB2 gag-expressing DCs to expand functional HIV-specific CD8(+) T cells. However, although most of the patients had detectable gag-specific CD8(+) T cell responses, no significant differences in the level of expansion of functional CD8(+) T cells could be demonstrated when comparing conventional or immune-modulated DCs expressing IL-12p70. This result which goes against expectation may lead to a re-evaluation of the need for IL-12 expression by DCs in order to improve T-cell responses in HIV-1-infected individuals. en
dc.language English en
dc.subject Viral diseases en
dc.subject HIV-1 en
dc.subject AIDS en
dc.subject Virus replication en
dc.subject Control en
dc.subject Therapy en
dc.subject Immune response en
dc.subject Gag en
dc.subject Interleukin-12p70 en
dc.subject Dendritic cells en
dc.subject T cells en
dc.subject mRNA en
dc.subject CD8-positive-T-lymphocytes en
dc.title Interleukin-12p70 expression by dendritic cells of HIV-1-infected patients fails to stimulate gag-specific immune responses en
dc.type Article-E en
dc.citation.jtitle Clinical and Developmental Immunology en
dc.citation.volume 184979 en
dc.citation.pages 1-11 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/22844321
dc.citation.jabbreviation Clin Dev Immunol en


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