Institute of Tropical Medicine Antwerp
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Interleukin-12p70 expression by dendritic cells of HIV-1-infected patients fails to stimulate gag-specific immune responses

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Show simple item record Van Gulck, E. Cools, N. Atkinson, D. Bracke, L. Vereecken, K. Vekemans, M. Van Tendeloo, V. F. I. Berneman, Z. N. Vanham, G. 2012-10-04T15:40:51Z 2012-10-04T15:40:51Z 2012
dc.identifier.issn 1740-2522
dc.identifier.other ITG-B1B
dc.identifier.other ITG-B3B
dc.identifier.other ITG-B4B
dc.identifier.other ITG-B5B
dc.identifier.other ITG-C6A
dc.identifier.other ITG-BLA
dc.identifier.other MULTI
dc.identifier.other DBM
dc.identifier.other U-VIROL
dc.identifier.other U-TRAVC
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other FTA
dc.identifier.other Abstract
dc.identifier.other UPD52
dc.description.abstract A variety of immune-based therapies has been developed in order to boost or induce protective CD8(+) T cell responses in order to control HIV replication. Since dendritic cells (DCs) are professional antigen-presenting cells (APCs) with the unique capability to stimulate na‹ve T cells into effector T cells, their use for the induction of HIV-specific immune responses has been studied intensively. In the present study we investigated whether modulation of the activation state of DCs electroporated with consensus codon-optimized HxB2 gag mRNA enhances their capacity to induce HIV gag-specific T cell responses. To this end, mature DCs were (i) co-electroporated with mRNA encoding interleukin (IL)-12p70 mRNA, or (ii) activated with a cytokine cocktail consisting of R848 and interferon (IFN)-?. Our results confirm the ability of HxB2 gag-expressing DCs to expand functional HIV-specific CD8(+) T cells. However, although most of the patients had detectable gag-specific CD8(+) T cell responses, no significant differences in the level of expansion of functional CD8(+) T cells could be demonstrated when comparing conventional or immune-modulated DCs expressing IL-12p70. This result which goes against expectation may lead to a re-evaluation of the need for IL-12 expression by DCs in order to improve T-cell responses in HIV-1-infected individuals. en
dc.language English en
dc.subject Viral diseases en
dc.subject HIV-1 en
dc.subject AIDS en
dc.subject Virus replication en
dc.subject Control en
dc.subject Therapy en
dc.subject Immune response en
dc.subject Gag en
dc.subject Interleukin-12p70 en
dc.subject Dendritic cells en
dc.subject T cells en
dc.subject mRNA en
dc.subject CD8-positive-T-lymphocytes en
dc.title Interleukin-12p70 expression by dendritic cells of HIV-1-infected patients fails to stimulate gag-specific immune responses en
dc.type Article-E en
dc.citation.jtitle Clinical and Developmental Immunology en
dc.citation.volume 184979 en
dc.citation.pages 1-11 en
dc.citation.jabbreviation Clin Dev Immunol en

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