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Tsetse salivary gland proteins 1 and 2 are high affinity nucleic acid binding proteins with residual nuclease activity

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dc.contributor.author Caljon, G.
dc.contributor.author De Ridder, K.
dc.contributor.author Stijlemans, B.
dc.contributor.author Coosemans, M.
dc.contributor.author Magez, S.
dc.contributor.author De Baetselier, P.
dc.contributor.author Van Den Abbeele, J.
dc.date.accessioned 2012-12-14T16:36:37Z
dc.date.available 2012-12-14T16:36:37Z
dc.date.issued 2012
dc.identifier.issn 1932-6203
dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0047233
dc.identifier.other ITG-B1B
dc.identifier.other ITG-B2B
dc.identifier.other ITG-B4A
dc.identifier.other ITG-BLA
dc.identifier.other DBM
dc.identifier.other U-VPROT
dc.identifier.other U-ENTOM
dc.identifier.other JIF
dc.identifier.other DOI
dc.identifier.other FTA
dc.identifier.other E-only
dc.identifier.other Abstract
dc.identifier.other UPD53
dc.identifier.uri http://hdl.handle.net/10390/7269
dc.description.abstract Analysis of the tsetse fly salivary gland EST database revealed the presence of a highly enriched cluster of putative endonuclease genes, including tsal1 and tsal2. Tsal proteins are the major components of tsetse fly (G. morsitans morsitans) saliva where they are present as monomers as well as high molecular weight complexes with other saliva proteins. We demonstrate that the recombinant tsetse salivary gland proteins 1&2 (Tsal1&2) display DNA/RNA non-specific, high affinity nucleic acid binding with K(D) values in the low nanomolar range and a non-exclusive preference for duplex. These Tsal proteins exert only a residual nuclease activity with a preference for dsDNA in a broad pH range. Knockdown of Tsal expression by in vivo RNA interference in the tsetse fly revealed a partially impaired blood digestion phenotype as evidenced by higher gut nucleic acid, hematin and protein contents. en
dc.language English en
dc.subject Protozoal diseases en
dc.subject Trypanosomiasis, African en
dc.subject Sleeping sickness en
dc.subject Trypanosoma brucei en
dc.subject Vectors en
dc.subject Tsetse flies en
dc.subject Glossina morsitans morsitans en
dc.subject Entomology en
dc.subject Vector biology en
dc.subject Bloodmeal en
dc.subject Digestion en
dc.subject Salivary glands en
dc.subject Endonucleases en
dc.subject Proteins en
dc.subject Nucleic acids en
dc.title Tsetse salivary gland proteins 1 and 2 are high affinity nucleic acid binding proteins with residual nuclease activity en
dc.type Article-E en
dc.citation.issue 10 en
dc.citation.jtitle PLoS ONE en
dc.citation.volume 7 en
dc.citation.pages e47233 en
dc.identifier.pmid http://www.ncbi.nlm.nih.gov/pubmed/23110062
dc.citation.jabbreviation PLoS ONE en


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